MEMCURV · Dynamic control of membrane curvature and its role in membrane protein assembly
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2013-02-01 → 2015-01-31
- Финансиране от ЕС
- 278 807 €
- Участници
- 1
- Схема
- MC-IIF
Линиите свързват координатора с партньорите.
Накратко на български
Липидните мембрани се изследват чрез създаване на повърхности с микроскопични изпъкналости и вдлъбнатини, които променят тяхната кривина. Това помага за разбирането на взаимодействието между мембраните и подложките, както и на начина, по който се подреждат протеините в тях.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Dynamic control of membrane curvature and its role in membrane protein assembly
During this year the first 2 phases of the project took place: (1) Construction of patterned substrate, and (2) Study of physical properties of supported lipid bilayers. The main goal of the 1st phase was to create substrates with a variety of three-dimensional patterns, and then to create local curvature changes in membrane by micro-patterning the underlying substrate. To this end, we had used two substrate-patterning techniques: colloidal lithography and electron beam lithography. Colloidal lithography is to deposit polystyrene particles with different diameters combined with thermal modification of radius of curvature. Using the similar strategy, we developed to make negative curvature with dissolving the polystyrene beads contained in the agarose layer. We also used electron beam (e-beam) lithography to make curved features in the nanometer range that can be highly repetitive and/or vary spatially. We had constructed kinds of bump and hole arrays with different periods and diameters. The 2nd phase focused on the properties of supported lipid bilayers with the goal to acquire deep understanding of the interaction of lipid bilayers with substrate. The 1st dealt with the preparation of lipid bilayers on flat mica surface, determining their physical properties, particular the mechanical properties. The results of these phase are included in the deliverable titled " work progress of curvature". A summary of the progress of the researcher training activities: The short-term objectives outlined in the "Career Development Plan" include: (i) research results, (ii) research skills and techniques, (iii) research management, and (iv) communication skills.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Changes in the curvature of the cell membrane play an important role in many important biological processes, including cell division, vesicle trafficking, and neurotransmission. These changes in morphology are not just a passive consequence of other events in the cell, but can also drive the action of other proteins. For example, changes in curvature are thought to affect protein binding, the gating kinetics of ion channels, and to direct the localisation of proteins in vivo.Although current methods can measure the presence of such interactions, they cannot directly measure real-time changes in membrane curvature or correlate individual protein binding events with changes in curvature. Dr Junhong Lu has a proven track record in Biological Atomic Force Microscopy. We propose to combine his expertise with new synthetic mimics of the cell membrane recently produced in the laboratory of Dr Mark Wallace at Oxford University. This combination of methods will enable us to manipulate and characterize the curvature of the cell membrane, and study its effects on protein function using single-molecule methods.
Оригинален текст от CORDIS (на английски).
Участници
- THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD · OxfordКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз