FP7Индивидуална стипендия2012–2014

ROMEO · ROle of crustacean zooplankton on prokaryotic community composition in the MEsopelagic Ocean

7РП — „Хора“ (Действия „Мария Кюри“)

Период: 2012-04-03 → 2014-04-02
Финансиране от ЕС: 180 191 €
Участници: 1
Схема: MC-IEF

Линиите свързват координатора с партньорите.

Накратко на български

Бактериите в червата на малките ракоподобни (копеподи) в Атлантическия океан се сравняват с тези в заобикалящата ги вода. Това помага да се разбере как тези организми влияят върху състава на микробите в дълбоките слоеве на океана.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

ROle of crustacean zooplankton on prokaryotic community composition in the MEsopelagic Ocean

The aim of the project was to determine the influence of metazoan zooplankton exhibiting diel vertical migration on the prokaryotic community in the Atlantic Ocean. Therefore, copepods’ samples were collected during two cruises (MEDEA I & II) in the North Atlantic Ocean. In order to address the main research questions, we used 454-pyro-sequencing of the 16S rRNA gene to obtain a detailed overview on the prokaryotic community composition associated with copepods’ gut (of different species) in the mesopelagic layers and to compare it with that of the ambient waters. Thus, the sequencing data obtained from the MEDEA I cruise samples was used to investigate the differences between the bacterial community composition associated with two orders of copepods (Cyclopoida and Calanoida) and the bacterioplankton community collected from the two boundary depth layers (~750 m, 100 m depth) at the same location as the copepods were collected. Our data showed significant differences between the bacterial communities associated with the dominant copepods families (Calanoida, Centropagidae; Calanoida, Clausocalanidae; Cyclopoida, Corycaeidae; Cyclopoida, Oncaeidae; Cyclopoida, Lubbockiidae) and the ambient water. Bacilli and Actinobacteria dominated the copepod-associated community and Alphaproteobacteria, Deltaproteobacteria and Synechococcus dominated the free-living community. However, the presence of shared bacterial OTUs between these two distinct habitats suggests a dynamic exchange of bacteria from seawater and copepods. Taken together, our results support the hypothesis that the interior and exterior surfaces of copepods provide a specific niche with a strong selective pressure for bacteria.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The ROMEO proposal aims at linking aspects of two scientific fields, traditionally separated, zooplankton ecology and microbial oceanography. ROMEO will investigate the influence of zooplankton activity on the prokaryotic community composition and activity in the open North Atlantic. During a four-week expedition in North Atlantic, the dominant crustacean zooplankton will be collected and the community composition of the resident and the transient gut prokaryotes determined during their nocturnal feeding in the surface waters and at daytime when they are residing at mesopelagic depths. Focus will be put on the two dominant calanoid copepod species in the North Atlantic, Calanus finmarchicus and C. helgolandicus. Also, the prokaryotic community and activity will be assessed in the fecal pellets and the successional changes in the community composition and activity followed during the decomposition of the fecal material. For the determination of the prokaryotic community composition, next generation sequencing will be applied on a metagenomic and –transcriptomic level. The hetero- and the autotrophic activity of the archaea will be differentiated from that of the bacteria using selective inhibitors, erythromycin for bacteria, diphtheria toxin for archaea. Bulk activity measurements as well as single-cell analyses will be performed and compared with the activity patterns in the ambient waters. Overall, the project will involve a substantial amount of bioinformatics work, which is the main training part of the researcher in ROMEO. The project will lead to an advancement of our understanding of the interaction between the main food source for North Atlantic fish, the calanoid zooplankton, and the main drivers of the biogeochemical cycles, the prokaryotes. Therefore, the expected results of this project will have impact on our understanding on the interactions between two different functional groups of organisms, playing major roles in the functioning of pelagic systems.""

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT WIEN · WienКоординаторАвстрия