PHEHETBACBIOFILM · Phenotypic heterogeneity in Bacillus subtilis biofilms
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2012-10-01 → 2016-09-30
- Финансиране от ЕС
- 100 000 €
- Участници
- 1
- Схема
- MC-CIG
Линиите свързват координатора с партньорите.
Накратко на български
Бактериите Bacillus subtilis се изследват, за да се разбере как различният метаболизъм на клетките влияе върху развитието на биофилмите, например при производството на защитен слуз. Това помага за по-доброто разбиране на устойчивостта на микробите в медицината и биотехнологиите.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Phenotypic heterogeneity in Bacillus subtilis biofilms
The “PheHetBioBacillus” project focuses on bacterial growth in complex communities and the impact of metabolic heterogeneity. In nature, most microbes exist in surface-attached sedentary communities known as biofilms. These communities provide a simple example of microbial complexity: certain cells residing in a biofilm deliver cooperative traits, like the production and secretion of extracellular polysaccharides that benefit the whole biofilm population as they improve biofilm resistance; however the biofilm matrix producing individuals have a delayed growth. In the tightly packed biofilms, bacteria stick together, surrounded by exopolymeric substances that protect them from unfavorable conditions and environmental stresses. Due to its remarkable resistance features, inhibition or promotion of biofilm development receives substantial attention in both health sciences and biotechnology. To better understand biofilm development, one can study the metabolism of different cell types within such a complex community. In this project, we have gained better understanding how the metabolic heterogeneity influences biofilm development. This grant facilitates the study of these aims above: the laboratory of Terrestrial Biofilms Group was established at the Friedrich Schiller University in Jena, Germany, where the project is carried out. During this project, various molecular microbiology and microscopy tools have been developed and used to study bacterial interactions and evolution during biofilm development. These techniques are now routinely used in our group to unravel how bacterial metabolism influences biofilm development. The project successfully identified the impact of metabolic heterogeneity on biofilm development and fulfilled all objectives of the proposed project. The output of the established group is exemplified by the numerous peer reviewed scientific publications and huge set of invited presentations. Project website: https://sites.google.com/site/terrestrialbiofilms/phehetbacbiofilm
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Biofilms are environmentally relevant lifestyles of microorganisms. Biofilms are usually isolated from surfaces or interfaces, where cells grow together, differentiate, produce a matrix and communicate through the matrix. This matrix also protects them from environmental insults, such as antimicrobials and several other stress conditions. Biofilms possess high complexity that is changing in time and space. To achieve such a differentiation cells enter various phenotypic pathways. Several studies present example how phenotypic heterogeneity (subpopulation of cells harbouring same genotype but expressing different sets of genes therefore showing diverged phenotype) plays a role during the development of biofilms. However, most of these studies concentrate on the genes coding for enzymes involved in the production of the biofilm matrix. Therefore studies that concentrate on the genes and regulation of genes that are involved in development of biofilm structures are desired. In this proposal, I will concentrate on the interplay between different cell types present in the biofilm and the role of heterogeneity in the development of biofilm structures and maturation. This proposal aims to unravel how the structure development is modulated in B. subtilis biofilms, what is the role of the cell growth strategies, the cell-cell communication, local cell level environment and gene expression in biofilm formation.The main objectives include (1) the development of an experimental system for bacterial biofilms, where heterogeneity in metabolic status of the cells can be followed; (2) the generation of a novel theoretical model for B. subtilis biofilms; (3) the isolation of growth yield favouring phenotypes; (4) detection of heterologously expressed transcripts at the single cell level. These objectives contain state of the art molecular biology techniques (fluorescent reporters), recently developed methods(single cell transcriptome) and novel approaches.
Оригинален текст от CORDIS (на английски).
Участници
- FRIEDRICH-SCHILLER-UNIVERSITÄT JENA · JENAКоординаторГермания
Връзки
Данни: CORDIS, © Европейски съюз