Benthic Ciliates · The Biodiversity, Systematics and Guide to the Identification of Marine Benthic Ciliates
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2012-08-15 → 2013-08-14
- Финансиране от ЕС
- 15 000 €
- Участници
- 1
- Схема
- MC-IIFR
Линиите свързват координатора с партньорите.
Накратко на български
Морските дънни цилиати (реснички) се изучават чрез анализ на видовете им в различни седименти, като пясъчни плажове и солени блата. Това помага за по-доброто разпознаване на тези организми чрез създаването на молекулярни данни и онлайн ръководство за идентификация.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
The Biodiversity, Systematics and Guide to the Identification of Marine Benthic Ciliates
The Benthic Ciliates project dealt with three main tasks during the return phase: 1) To increase knowledge of species diversity of marine benthic ciliates. 2) To provide molecular sequence data for selected taxa. 3) To develop a web-based guide to the identification of marine benthic ciliates. * Sample collection of different sediments (sandy beach, muddy flats, salt marshes etc.) was mainly undertaken during 10 field trips to various locations along the southern and northeast coasts of China. After isolation, raw and/or pure cultures were established. Live ciliates were observed and photographed. Ciliates were stained using silver impregnation methods in order to reveal infraciliature and silverline system. A total of 112 permanent slides were prepared. Based on morphological and statistical data from live and stained specimens, 45 species of marine benthic ciliates have been identified since the beginning of the return phase. Morphogenesis was investigated for 4 species. * DNA extractions and PCR amplification have been carried out for 30 species and sequence data were obtained for 15 species. Phylogenetic analyses have been carried out for 10 species. * A literature search was carried out and data assembled for the construction of a web-based guide to marine benthic ciliates. Data collection was completed. The structure of the guide was constructed within a Scratchpad. With the coming of the new version of Scratchpad, our old site http://s1.ciliate.myspecies.info will have to be upgraded to the new one. This relocation commences on 10 March 2014. Data imputing and updating has not be completed yet. The significant findings included: 1. It is the only one that utilises all three of the main on which the modern taxonomy of ciliates is based, namely morphological, morphogenetic and molecular. This has so far resulted in the discovery of three new genera, 11 new (sub) species and 19 new records for China. Meanwhile, morphogenetic modes of four species are revealed for the first time. The current work therefore constitutes a significance advance in knowledge of Chinese biodiversity of marine benthic ciliates and will help China to fulfil their obligations to the Convention on Biological Diversity. 2. Molecular sequence data are first available for 30 species, several of which were previously of uncertain taxonomic affinity. The present study therefore helps to determine their systematic position and thus provide the basis for a more robust taxonomy. In other cases the molecular data will allow the genetic relatedness between morphospecies from geographically distant localities to be assessed thereby informing the debate on whether such taxa are cosmopolitan or are geographically restricted.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Ciliated protozoa (ciliates) are important in terms of their diversity, ecology, pathogenesis and as indicators of environmental quality. Marine benthic ciliates, however, are rather poorly known due to a lack of modern treatments and taxonomic expertise. The objectives of this project are: (1) to increase knowledge of local and global species diversity. A faunistic study of benthic ciliates from UK coastal waters will be carried out using classical microscopy and modern molecular methods. The data for selected UK and Chinese species will be compared in order to assess their geographical distributions. (2) To increase understanding of phylogenetic relationships among ciliates and thus help stabilise the Tree of Life. For this, morphogenetic and molecular data for selected taxa will be used in phylogenetic analyses. (3) To develop a web-based guide to the identification of marine benthic ciliates. This will be constructed within a Scratchpad (http:// scratchpads.eu/) which is a tool developed at the NHM that allows for species descriptions to be made available on the web. The present project will extend this functionality by adding an interactive key based around structured descriptive data. New taxa in the correct format will automatically add them to the key without further intervention. Dr Hu is a top-class researcher from China who specialises in marine benthic ciliates. Expertise in this field is increasingly rare in Europe and has led to a lack of understanding of the importance of benthic ciliates in marine microbial ecology and their contribution to species diversity. This project will help to: enhance Dr Hu's reputation as a leading authority on marine benthic ciliates; increase his network of European contacts; by developing the open-access web-based guide, increase opportunities for European researchers to collaborate with Dr Hu and colleagues; improve his standard of English thus facilitating better communication with potential collaborators in Europe.
Оригинален текст от CORDIS (на английски).
Участници
- Ocean University of China · QINGDAOКоординаторКитай
Връзки
Данни: CORDIS, © Европейски съюз
