GENDER · Molecular mechanisms monitoring sex determination in Arabidopsis thaliana
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2013-04-01 → 2015-03-31
- Финансиране от ЕС
- 202 406 €
- Участници
- 1
- Схема
- MC-IEF
Линиите свързват координатора с партньорите.
Накратко на български
Генните фактори WIP при растението Arabidopsis thaliana определят как се развива растението и как се формира неговият пол. Разбирането на тези механизми помага за по-доброто познаване на развитието на тиквите и пъпешите.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Molecular mechanisms monitoring sex determination in Arabidopsis thaliana
The main purpose of our project was to use a combination of genetic and genomic approaches, more easily implemented in the model plant A. thaliana, to determine the molecular function of family of transcription factors involved in sex determination in melon, the WIP genes. The final goal was to increase our knowledge on about their function in A. thaliana and propose new concepts that will be translated then to cucurbit plants. To attend this objective we have first started our analysis by performing GUS expression analysis to determine the expression profile of each WIP gene in A. thaliana seedlings. Base on the results obtained we have suggested a redundant function between WIP proteins in control of plant development. In order to confirm this hypothesis we generated a combination of double and triple wip mutants and analyzed plants for phenotype alterations. We generated ‘omics’ data of WT and mutant lines impaired on WIP functions. Integration of genetic and omics data led to a molecular model that explains how the WIP proteins control plant development.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
In higher plants, sex determination is usually controlled by the genotype and they can be modified by various environmental factors and/or by hormones present in the plant. The mechanism by which this process is controlled in plants is still poorly understood.In melon, sex determination is governed by the identity of two genes, andromonoecious (a) and gynoecious (g). The gene a encodes for an ethylene biosynthesis enzyme, CmACS-7, that repress stamen development in female flowers. The g gene encodes for a transcription factor, CmWIP1. CmWIP1 represses indirectly CmACS-7 expression to allow stamen development. The model suggests that the two genes interact to control the development of male, female and hermaphrodite flowers.CmWIP1 gene encodes for a nuclear protein that is conserved throughout the plant kingdom and defines a new subfamily of plant zinc finger proteins. In Arabidopsis thaliana genome 6 WIPs have been identified. However, none of the WIP proteins has been functionally characterised.The goal of this proposal is to study the expression and regulation of WIP proteins in A. thaliana. We will use as a starting point the information obtained from the sequence analysis of CmWIP1 in melon to understand if the function of this protein is conserved between melon and A. thaliana. Preliminary experiments shown that ectopic expression of the CmWIP1 gene can determine the sex of A. thaliana plants. Using a combination of genetic and genomic approaches, more easily implemented in the model plant A. thaliana, we will study the involvement of WIP proteins in plant sex determinism and development. The finding and the concepts that will be gathered on the A. thaliana model system will be transferred to melon at later stage of the project. Understanding how different sexual systems in plants have evolved, we can then begin to understand how sex in general evolves.""
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT · ParisКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз