NONCODOWN · Optimizing Antisense Oligonucleotides for Efficient and Specific Knockdown of Nuclear Non-Coding RNA
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2013-06-01 → 2017-05-31
- Финансиране от ЕС
- 100 000 €
- Участници
- 1
- Схема
- MC-CIG
Линиите свързват координатора с партньорите.
Накратко на български
Специфични молекули, наречени антисенсови олигонуклеотиди, се тестват за ефективно блокиране и разграждане на некодираща РНК в ядрото на клетката. Това помага за разработването на по-прецизни инструменти за управление на регулаторните процеси в клетките на бозайниците.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Optimizing Antisense Oligonucleotides for Efficient and Specific Knockdown of Nuclear Non-Coding RNA
The past decade has seen a dramatic increase in discovery of noncoding RNA with functional regulatory roles in the nucleus of mammalian cells. Yet tools to manipulate these nuclear noncoding RNAs remain inadequate. This project has taken a multi-pronged approach to technology development for silencing nuclear RNAs. (1) We have developed a series of optimized antisense oligonucleotides for targeting and degrading structured RNAs by containing a partial sequence of PNA and part of DNA, joined by a conformationally locked nucleotide. (2) We have compared a number of chemical designs for silencing a nuclear-retained RNA transcript, including siRNAs, single-stranded siRNAs, and LNA-modified antisense oligonucleotides (ASOs). We found that the ASOs were by far the most potent for this task. (3) We have screened chemically modified oligonucleotides, including Locked Nucleic Acid and Peptide Nucleic Acid, for blocking the action of a bacterial regulatory ncRNA, and identified potent inhibitors.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The past decade has seen a dramatic increase in discovery of noncoding RNA with functional regulatory roles in the nucleus of mammalian cells. Yet tools to manipulate these nuclear noncoding RNAs remain inadequate. This proposal will develop optimized antisense oligonucleotides for targeting and degrading structured RNAs in the nucleus. This will be carried out by using both the best of what already exists in the field of nucleic acid chemistry (e.g. locked nucleic acid and the gapmer approach) and innovative new designs (e.g. CuAAC-derived PNA-DNA-LNA chimeric oligomers for degrading structured RNA targets).
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITY OF SOUTHAMPTON · SOUTHAMPTONКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз