H2020Индивидуална стипендия2016–2018

PERFECTION · Probing mechanisms of pathogen effector recognition by plant Resistance proteins to elevate defence gene activation

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-04-01 → 2018-03-31
Финансиране от ЕС: 183 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Имунните рецептори (R-протеини) при растенията се изследват, за да се разбере как те разпознават патогени и активират защитните гени. Това помага за намаляване на загубите от реколтата чрез по-добро разбиране на механизмите за растителна защита.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Probing mechanisms of pathogen effector recognition by plant Resistance proteins to elevate defence gene activation

Plant diseases can lead to up to 40% crop losses every year. To help provide sustainable solutions for reducing the diseases, we need to improve our mechanistic understanding of how plant immunity has been switched ON. Resistance (R) proteins, a group of immune receptors that can confer resistance to a broad spectrum of pathogens, are one of the most important genetic components in plant immunity. The first Resistance gene was cloned 25 years ago, but the detailed mechanism by which activation of Resistance protein upon perceiving pathogenic molecules leads to reprogramming of defense genes remains largely unknown. In sum, my PERFECTION project aimed to address this knowledge gap by answering several important questions: 1) can I find the composition of or novel components in plant immunity mediated by R proteins, by generating novel bio-materials? 2)whether the R proteins regulate downstream defense genes directly? 3)can I use sequencing methods to understand the gene regulation mechanisms of how defense genes are regulated? The importance of this project can be addressed by developing new tools for studying the mechanisms of how plant R proteins signal to downstream defense genes; generating new metadata as an open-access resource for the wider community, thereby promoting studies of defense gene regulation in plant immunity; and generating novel bioinformatic tools for data analysis.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Plant cells tightly regulate gene transcription in response to a changing environment. Stresses, such as pathogen encounters, lead to dramatic transcriptional reprogramming to favor defence activation over basal cellular functions. For effective defence, cells must rapidly alter defence gene mRNA abundance. How extracellular and intracellular recognition of plant pathogens trigger appropriate changes in host mRNA abundance is poorly understood. Upon recognition of pathogen effectors, resistance proteins activate plant defence by unknown mechanisms. My host lab recently reported that the Arabidopsis Resistance gene pair RPS4/RRS1-R elevates expression of certain defence genes, such as those required for salicylic acid biosynthesis, within four hours of detecting PopP2 effector in Arabidopsis. The main goal of this proposal is to understand how effector recognition by RPS4/RRS1-R leads to rapid defence gene induction. We will test the hypothesis that RPS4/RRS1-R proteins directly interact with gene loci that are activated during this process. I will use transgenic Arabidopsis that carry a single genome locus with independently epitope-tagged RPS4, RRS1 and other defence-implicated proteins to investigate: (1) changes in composition of the RPS4/RRS1-R protein complex upon effector recognition using mass spectrometry; (2) effector-induced changes in association of the RPS4/RRS1-R proteins with induced genes using chromatin immunoprecipitation; (3) changes in chromatin status at induced gene regions correlated with gene induction and activation of defence by RPS4/RRS1-R. From this project, I will rigorously test an important model, namely that plant immune receptor complexes directly mediate transcriptional reprogramming of defence genes through chromatin changes upon recognition of effectors. I bring highly complementary expertise in genetics and transcriptional regulation of basal defence from China to Canada and now to TSL that is essential for this project’s success.

Оригинален текст от CORDIS (на английски).

Участници

THE SAINSBURY LABORATORY · NorwichКоординаторОбединеното кралство