H2020Индивидуална стипендия2015–2017

BIOeSPUN scaffolds · Bioactive glass in electrospun matrices: functionalised smart scaffolds for interface tissue engineering applications

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-06-01 → 2017-05-31
Финансиране от ЕС: 159 461 €
Участници: 1
Схема: MSCA-IF-EF-ST

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Накратко на български

Многослойни структури от биоактивно стъкло и полимери се разработват за възстановяване на стави, например при увреждания на хрущяла и костта. Тези материали имитират естествената тъкан и помагат за лечението на хронични заболявания на опорно-двигателния апарат.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Bioactive glass in electrospun matrices: functionalised smart scaffolds for interface tissue engineering applications

“BIOeSPUN scaffolds” project aims the fabrication and characterization of novel types of multilayered scaffolds suitable for interface tissue engineering (ITE) applications, in particular for osteochondral segment regeneration. Osteochondral lesions are defects or diseases which usually involve the cartilage, bone or the interface cartilage-to-bone tissue and they can occur in all the joints of the human body, like knee, shoulder, elbow, wrist, and hip. The causes of these articular defects could be traumatic or due to aging-related disease, involving young, adult and older patients. For this reason an effective and long lasting treatment is needed. It is fundamental to mention that articular joint disease (and generally musculoskeletal conditions) is included in the list of major and chronic disease (MCDs), defined as pathologies affecting at least 50 per 100000 people, causing together 87% deaths in EU. In this framework, interface tissue engineering (ITE) offers an innovative approach for the regeneration of interface tissues, like osteochondral segment. ITE aims the fabrication of scaffolds able to mimic the complexity and anisotropy of the native tissues. “BIOeSPUN scaffolds” project reported the successful fabrication and characterization of multilayered graded scaffolds for the osteochondral segment regeneration. The electrospinning technique was selected for the fabrication of fibrous scaffolds, mimicking the morphology of the native extra cellular matrix. Benign solvents were used for the electrospinning process to avoid the use of toxic harsh solvents, having advantages in terms of avoidance of toxic solvents residuals in the obtained scaffolds. The use of benign solvents for the electrospinning is innovative and challenging because often these solvents require additional process parameters optimization. The electrospinning technique was combined with foam replica method and dip-coating for the fabrication of scaffolds having morphological and compositional gradients among the different layers. Cell biology studies were performed on the obtained multilayered scaffolds. The additional value of this project was represented by the training activities which allow the enrolled researcher to improve her skills and to complete her interdisciplinary profile.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The project aims to fabricate and characterise novel type of multilayered scaffolds suitable for interface tissue engineering applications, in particular for osteochondral segment regeneration. Osteochondral defects imply injury in cartilage, bone and bone-cartilage interface tissues, characteristic of all the joints of human body, its causes could be both traumatic and due to aging-related pathologies. The project will be focused on the integration of several scaffold fabrication techniques for the development of novel electrospun multilayered scaffolds. In particular, considering the well-known effects of bioactive glass on osteogenesis, angiogenesis and its antibacterial activity, electrospun bioactive glass mats will be fabricated. Moreover, bioactive glass particles will be dispersed in a polymeric solution before the electrospinning for the fabrication of bioactive glass-doped mats. These two kind of scaffold will be used for the fabrication of the multilayered structures. Innovative solutions will be adopted for the obtainment of the stratified samples, integrating different technologies as the electrospinning, freeze-drying and foam replica method. The training of the researcher in the host Institution will be focus on the development of skills related to cell culture management and in particular on cell culture for tissue engineering. The training will start with cell viability tests and cell seeding on several kind of scaffolds. Several cell lines will be used and it will be also investigated stem cells differentiation. The use of co-culture systems and dynamic cell culture will also be evaluated and eventually applied to the multilayered scaffolds. An innovative approach will be used in the investigation of scaffold mechanical properties, in fact mechanical tests will be performed on the multilayered samples and on each single layer to evaluate the deposition of ECM on the seeded scaffolds and how it affects scaffold mechanical properties.

Оригинален текст от CORDIS (на английски).

Участници

FRIEDRICH-ALEXANDER-UNIVERSITAET ERLANGEN-NUERNBERG · ErlangenКоординаторГермания