H2020Индивидуална стипендия2015–2017

TWISTER · ToWards the Identification of Sesquiterpene TransportERs

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-08-01 → 2017-07-31
Финансиране от ЕС: 160 800 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Транспортерите на сесквитерпени при растението Artemisia annua се изследват, за да се разбере как вещества като бета-кариофилен се отделят от клетките. Това е важно, тъй като тези съединения се използват в медицината за борба с малария, рак и различни видове болки.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

ToWards the Identification of Sesquiterpene TransportERs

In plants, trichomes are small protrusions of the epidermis present on the surface of aerial organs. Some of them, namely glandular trichomes, stand for a chemical barrier against pathogens, synthesizing and excreting large amounts of secondary (or specialized) metabolites such as terpenes. Because of their biochemical properties, some of these metabolites are used by humans in pharmaceutical, fragrance, and food additive industries. In Artemisia annua, glandular trichomes have been extensively studied since they are known to produce the sesquiterpene lactone, artemisinin, whose derivatives are extremely potent anti-malarial agents and appear to be effective drugs against other diseases including cancers. Besides, A. annua bears another type of trichomes, which so far has received little attention. These T-shaped trichomes are involved in the synthesis and the secretion of another sesquiterpene, ß-caryophyllene, which is part of the volatile cocktail playing roles in recruiting natural enemies of herbivores. Moreover, ß-caryophyllene was shown to be involved in the modulation of inflammatory and neuropathic pain responses. Despite the immense benefits of these compounds for plant defense and human health, molecular mechanisms leading to sesquiterpene secretion out of the cell are still undeciphered. However, it was recently shown that some Pleiotropic Drug Resistance (PDR) transporters, which belong to the ATP-Binding Cassette family, are involved in transporting diterpenes across the plasma membrane, leading to their excretion from Nicotiana tabacum and N. plumbaginifolia trichomes. Thus, other PDR transporters are expected to transport other types of terpenes such as sesquiterpenes. In A. annua, two PDR transporters, namely AaPDR1 and AaPDR2, have been shown to be specifically expressed in glandular and T-shaped trichomes. In this context, the overall objectives of this project were to investigate the putative role of AaPDR1 and AaPDR2 in sesquiterpene transport and decipher the functions of T-shaped and glandular trichomes in A. annua.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Plants synthesize a wide range of secondary metabolites, among them, terpenes. These molecules are notably produced in trichomes which are specialized structures present on the surface of aerial organs. Although the terpene biosynthetic pathways have been well deciphered, their transport across membranes remains poorly understood. This is for instance the case for sesquiterpenes such as artemisinin, an extremely potent anti-malaria drug. Despite the immense benefits of these compounds for plant defenses as well as for human health, no sesquiterpene transporters have yet been identified.It was recently shown that Pleiotropic Drug Resistance (PDR) transporters, which belong to the large ATP-Binding Cassette family, are involved in diterpene transport in Nicotiana tabacum trichomes. Thus, other PDR transporters could transport other types of terpenes such as sesquiterpenes.Recently, two PDR transporters of Artemisia annua, namely AaPDR1 and AaPDR2, have been shown to be specifically expressed in glandular and T-shaped trichomes, respectively. Interestingly, glandular trichomes are known to produce artemisinin while T-shaped trichomes have been shown to produce ß-caryophyllene. Thus, we can hypothesize that AaPDR1 and AaPDR2 transport dihydroartemisinic acid, the artemisinin precursor, and ß-caryophyllene, respectively.In order to examine this hypothesis, this project aims to express these transporters in N. tabacum BY2 cells and characterize their activity using toxicity and transport assays in whole cells as well as direct transport in plasma membrane vesicles. In case our hypothesis concerning the substrates is incorrect, we will rely on a transportomics approach, which consists of running transport assays using metabolites extracted from isolated trichomes as putative substrates. In parallel, as a side project, isolated glandular and T-shaped trichomes will be submitted to quantitative proteomic comparison in order to identify their respective metabolisms.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE CATHOLIQUE DE LOUVAIN · LOUVAIN LA NEUVEКоординаторБелгия