H2020Индивидуална стипендия2016–2018

MIP4CELL · Molecular Imprinting mediated regulation of cell behavior and related biomedical applications

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-03-01 → 2018-02-28
Финансиране от ЕС: 185 857 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Изследват се изкуствени матрици, които имитират естествената среда около клетката чрез променливи биоактивни връзки. Това помага при възстановяване на тъкани след раково лечение или при наранявания на гръбначния стълб и мозъка.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Molecular Imprinting mediated regulation of cell behavior and related biomedical applications

The applications of the research carried out in this project are found in medicine – in tissue engingeering, reproductive medicine and stem cell therapies. This refers to replacement of cells or tissue e.g. in cancer treatment due to loss of healthy cells hit by cytostatic agents or in brain or spinal cord injury. To produce the tissue or cells requires a medium mimicking the native environment around the cell and this is what this project aims to do. Cellular processes are hence crucially dependent on dynamic receptor-ligand interactions occurring at the interface between the cell membrane and the extracellular matrix (ECM) (J. Robertus, W. R. Browne, B. L. Feringa, Chem. Soc. Rev. 2010, 39, 354 – 378.) Changes in these interactions as a consequence of ECM remodeling, give rise to specific cell signaling and intracellular cascades. These processes are central in the physiology and pathological processes like tissue self-repair and tumorigenesis. As mimics of such dynamic interactions, artificial matrices with reversible display of bioactive ligands have attracted much attention. Surfaces capable of modulating cell-biomaterial interactions are commonly exploited for in-situ cell biology experimentation and in tissue engineering. Current methods to control reversible ligand presentation on biomaterial interfaces mainly rely on surface functionalization with reversible linkers (e.g., noncovalent or reversible covalent interactions) to which the bioactive ligand is tethered (Fig.1). For example, by means of host-guest chemistry, reversible covalent chemistry, molecular assembly or other multiple non-covalent interactions, the integrin-targeted cell adhesive peptide RGD (Arg-Gly-Asp) could be dynamically and reversibly immobilized on the biointerfaces to regulate cell adhesion behavior. These approaches towards simulating the reversible ligand presentation in a biological system have greatly promoted the development of dynamic biointerfaces and a new generation of artificial ECM materials. However, to date, only a few reversible linkage chemistries have been exploited and even fewer that can be geometrically adressed. The overall objective of this project was to develop a new dynamic and geometrically addressable methodology for biofunctionalization and apply it to reversible cell adhesion and tissue engineering.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Tissue engineering or regenerative medicine, with its ultimate aim of developing true human replacement parts, has recently made enormous progress. The field refers to the use of human cells to replace or regenerate tissues or organs. Ideally, biomaterials for tissue engineering should efficiently mimic functionalities of the natural extracellular matrix (ECM). Hence surfaces of biomaterials should be engineered for dynamic control of cell behaviour. The objective of this proposal is to exploit molecular imprinting as a dynamic methodology for biofunctionalization and tissue engineering. Building on our previous proof of concept demonstration of systems for cell sheet harvesting, we expect to hereby obtain dynamic biofunctionalization of surfaces and overcome limitations of currently used methodologies (e.g. ligand leaching in non-covalent approach, loss of bioactivity in covalent approach, and lack of means for dynamic control of cells adhesion in both). The specific objectives of the proposal are as follows:1) To establish bioimprinted hydrogels with thermo-responsive affinity to bioactive cell adhesive peptide as a generic platform for dynamically regulating cell adhesion and migration.2) To develop thermo-responsive DNA aptamer imprinted hydrogels for dynamic recognition of specific cells via cell-aptamer interactions, and using as a platform for capture and release tumor cells. Therefore, this proposal will mimic the in vivo dynamic characters of the ECM and allow to obtain controllable cell behaviours in artificial biomaterials, thus having potential applications in medical diagnostic and regenerative medicine.Bringing together a highly talented and creative experienced researcher, who has already contributed decisively to the field, with a leading research group in the field of molecular imprinting and biomedicine will allow for the aforementioned objectives to be successfully accomplished.

Оригинален текст от CORDIS (на английски).

Участници

MALMO UNIVERSITET · MALMOEКоординаторШвеция