H2020Индивидуална стипендия2015–2017

SELECT-A-PROTEIN · Mixed brushes of stimuli-responsive polymers for the selective adsorption of proteins

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-10-01 → 2017-09-30
Финансиране от ЕС: 172 800 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Смесени полимерни слоеве се изследват за избирателно привличане на определени протеини чрез промяна на pH или солеността на средата. Това помага за подобряване на биосензорите, системите за доставка на лекарства и лабораторните анализи.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Mixed brushes of stimuli-responsive polymers for the selective adsorption of proteins

Protein adsorption at solid/liquid interfaces is a common phenomenon that plays a key role in many biomedical and biotechnological areas. It is a crucial event for the development of applications such as enzyme carriers, biosensors, drug delivery systems, immunological assays, cell culture substrates and so forth. On the other hand, non-specific protein adsorption on sensor surfaces or assay platforms is problematic because it degrades the analytical performances of the device. Therefore, the desire to predict, control, and manipulate protein adsorption on different surfaces has been the main driving force in this arena. Many different strategies have recently been developed in order to control protein adsorption. Most of them are based on the use of polymers at interfaces. Protein adsorption may be efficiently tuned using smart interfaces based on stimuli-responsive brushes. These systems are very promising for the development of numerous applications due to their ability to switch interaction forces between the brush and the surrounding environment. Mixed polymer brushes, composed of a protein adsorbing and of a protein-repellent polymer, are of particular interest due to their ability to finely control protein adsorption. The brush properties can be controlled by the amount of both polymers, polymer chain length, and by applying external triggers such as pH, ionic strength, etc. Based on this concept, we set two specific objectives: 1) developing a surface layer for the temporal control of protein adsorption, and 2) achieve the selective adsorption of proteins from a mixture of proteins in a stimuli-triggered manner. In this project, we built brushes using a combination of either a polyanion or a polycation with a protein-repellent polymer. The properties of these brushes were adjusted by different ratios of both polymers, different molar masses of the protein-repellent polymer, and ionic strength and pH changes in the surrounding medium. We have successfully achieved the goal of this research project during the past 2 years, with the development of two systems that allows one to selectively adsorb one protein from a mixture of proteins and to further completely desorb it from the surface. This opens perspectives for protein separation, diagnostic applications, etc

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Advanced materials are at the heart of many scientific fields, particularly in the biomedical and biotechnological areas. Many devices (e.g., biosensors, implants, catheters, and systems for drug delivery) would benefit from an effective control over molecular and cellular interactions at material surfaces. Main challenges in this field include the control of biomolecules (e.g., proteins, DNA) adsorption on different surfaces. Designing surfaces that allow proteins to be adsorbed in a controlled manner is particularly important and challenging. Many strategies were recently developed to spatially or temporally control protein adsorption.In this project, a novel strategy for selective protein adsorption from a mixture of proteins will be developed. This strategy will be based on the use of mixed polymer brushes sensitive to ionic strength and pH of the surrounding medium. The project rests on the following steps:i.The design of mixed polymer brushes whose properties will be tuned by adjusting pH and ionic strength of the medium. The influence of polymer properties (molecular weight, and degree of dissociation and conformation through pH and ionic strength variations) on protein adsorption will be studied.ii.The development of a procedure, based on time-of-flight secondary ion mass spectrometry (ToF-SIMS), to distinguish different proteins adsorbed together on model interfaces.iii.The use of this ToF-SIMS-based procedure to investigate the potential of the created mixed brushes to selectively adsorb one protein, at first from a mixture of two or three proteins, and then from a more complex medium.The results obtained in this work will be significant for material science and biomedical applications through the development of a robust procedure to prepare “smart” surfaces with novel properties to control protein adsorption.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE CATHOLIQUE DE LOUVAIN · LOUVAIN LA NEUVEКоординаторБелгия