H2020Индивидуална стипендия2015–2018

SymPathInfect · Symbiont-mediated defense of amoebae against Legionella pneumophila - molecular mechanisms and pathogen ecology

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-10-01 → 2018-09-24
Финансиране от ЕС: 178 157 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Взаимодействията между свободноживещите амеби, техните бактериални симбионти и патогена Legionella pneumophila разкриват как някои бактерии оцеляват вътре в амебите. Това помага за по-доброто разбиране на механизмите, които водят до огнища на заболявания, пренасяни чрез вода.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Symbiont-mediated defense of amoebae against Legionella pneumophila - molecular mechanisms and pathogen ecology

Bacterial pathogens are generally investigated in the context of diseases. Their environmental lifestyle and reservoirs are often neglected. This project aims to fill this gap by studying the interaction of free-living amoebae with their bacterial symbionts and the bacterial pathogen Legionella pneumophila. Free-living amoebae are ubiquitous protists and important predators of bacteria. As such they have a strong impact on the structure of microbial communities. Several bacteria developed strategies to survive digestion by amoebae and are even able to multiply within amoebae. This includes bacterial symbionts such as Protochlamydia amoebophila and Simkania negevensis as well as opportunistic pathogens like Legionella pneumophila, the causative agent of an atypical pneumonia called Legionnaire’s disease. Our goal is to shed light on the molecular and physiological interactions between amoebae, their symbionts and opportunistic human pathogens. This project contributes to a better understanding of the role of amoebae and other microorganisms in water-borne disease outbreaks.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Legionellae are opportunistic human pathogens infecting thousands of people annually in Europe. These bacteria are ubiquitous in many natural and man-made water systems, where they survive as free-living organisms in biofilms or intracellularly within a variety of protozoa. Free-living amoebae are the main route for spread and replication of legionellae in the environment, and infection of humans generally occurs via amoebae as vectors. Amoebae are frequently associated with bacterial endosymbionts, and recent data suggests that these symbionts interfere with replication of legionellae in amoebae thereby protecting the amoeba host from legionellae-induced lysis. The aim of this proposal is to further investigate this phenomenon of symbiont-mediated defense in protozoa, and to assess its implications for the ecology and transmission of legionellae. To shed light on the molecular and physiological interactions during infection of symbiont-containing amoebae with Legionella pneumophila, co-infection experiments will be performed and analyzed by state-of-the-art molecular methods including transcriptomics, proteomics and metabolomics, isotope profiling and chemical imaging techniques. Infection experiments will first be performed under controlled laboratory conditions to understand the interaction between amoeba, their bacterial symbionts and L. pneumophila, to analyse the molecular cross-talk, and to determine the mechanism of competition between the bacterial partners in this association. Subsequently, mesocosm experiments simulating environmental conditions will help to understand the impact of bacterial symbionts of amoebae on L. pneumophila spread and replication in the environment. Taken together, the comprehensive analysis of symbiont-mediated defense in amoebae will provide a new perspective on the ecology of L. pneumophila and lead to a better understanding of the role of amoebae and other microbes in water-borne disease outbreaks.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT WIEN · WienКоординаторАвстрия