H2020Индивидуална стипендия2016–2018

LABVaccFish · USE OF LACTIC ACID BACTERIA AS DELIVERY VEHICLE FOR ORAL VACCINATION OF FISH

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-01-04 → 2018-01-03
Финансиране от ЕС: 165 599 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Млечнокиселите бактерии се тестват като средство за орална ваксинация на карпи срещу вируса на пролетната виремия. Такъв метод чрез храната или водата може да намали разходите и стреса при животните при масовото им ваксиниране.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

USE OF LACTIC ACID BACTERIA AS DELIVERY VEHICLE FOR ORAL VACCINATION OF FISH

Since the seventies, aquaculture production has continued to grow with an average annual rate of 6.3% to reach its present 59.9 million tons of product. Despite the continuous growth of aquaculture output, outbreaks of viral diseases continue to be a significant limiting factor and the control of viruses is imperative to sustain future development of the aquaculture sector. In this project we addressed problems related to fish vaccination, with a special focus on DNA vaccination of carp against Spring Viraemia of Carp Virus. SVCV is one of the major threats to carp production in East Europe and Asia. In particular we evaluated the possibility to use Lactic acid bacteria (LAB) as vehicles to carry and/or deliver vaccine antigens to fish. LAB are generally regarded as safe (GRAS); some species of LAB are very well studied beneficial microbes (probiotics) present in the intestine of humans and animals (commensals), often used as probiotics in drinks such as Actimel or Yakult. Given their safety profile and health benefits in mammals, we decided to modify LAB, and analyse whether they could be used for oral or injection vaccination of fish. The proof of principle that LAB can be used for vaccination of fish can provide a novel and safe approach to fish vaccination. Since LAB could be given in the water or mixed with the feed it would provide a suitable route of mass vaccination of fish that would reduce costs and improve animal welfare by reducing stress due to handling

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

This project will provide a proof of principle that food-grade lactic acid bacteria (LAB) can be used as vehicle for oral vaccination of fish and will unravel the underlying mechanisms of memory formation in zebrafish. We will engineer Lactococcus lactis to deliver a DNA- or protein-based prototype vaccine against spring viraemia of carp virus (SVCV). The choice of virus and antigen is based on work showing that injection vaccination with DNA plasmids encoding the G protein of SVCV is highly successful. This vaccination route however, is less suitable for mass delivery and alternative strategies aiming at delivering the vaccine via the oral route are required. Food-grade lactic acid bacteria as vaccine vehicle are an attractive strategy to deliver vaccines at mucosal surfaces that has never been investigated in fish. We will use zebrafish as animal model to monitor real-time vaccine uptake and kinetics of immune responses. Already available knock-out and transgenic zebrafish lines expressing reporter genes under the control of cell- or cytokine-specific promotors will help dissect the contribution of innate and adaptive immune responses induced by vaccination. We will develop novel transgenic zebrafish lines for the controlled ablation of zebrafish cytotoxic T lymphocytes (CTL), to test the hypothesis that long term protective immunity to SVCV is mainly CTL-mediated. Altogether, this project will provide a proof of principle for the development of mucosal vaccines suitable for mass delivery in fish using commensal bacteria, supported by the unravelling of immune mechanisms important for memory formation using zebrafish as animal model. This project will familiarize the candidate with mucosal immunology using state-of-the-art techniques such as transgenesis and live microscopy in an animal model of high interest to the Life Sciences; this will promote his development as independent researcher and facilitate the acquisition of a stable research position in Europe

Оригинален текст от CORDIS (на английски).

Участници

WAGENINGEN UNIVERSITY · WageningenКоординаторНидерландия