H2020Индивидуална стипендия2015–2017

PFSEXOME · Identification of protein kinases and signalling pathways important for Plasmodium falciparum malaria transmission

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-04-01 → 2017-03-31
Финансиране от ЕС: 183 455 €
Участници: 2
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Протеиновите кинази и сигналите, които управляват превръщането на паразита Plasmodium falciparum в сексуални форми, се анализират чрез изследване на фосфопротеома. Това помага за разбирането на механизмите, чрез които маларията се предава от човек на човек.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Identification of protein kinases and signalling pathways important for Plasmodium falciparummalaria transmission

Malaria is a devastating disease caused by the apicomplexan parasite Plasmodium. Plasmodium falciparum represents the most severe and life-threatening form of human malaria, causing over 500,000 deaths per year. There is no efficient vaccine available and resistance to all currently used drugs reported. One of the major problems in eradicating human malaria is the transmission of the parasite sexual stages, the gametocytes. The molecular regulatory mechanisms important for the formation of gametocytes are poorly understood but there is evidence showing that phosphorylation of epigenetic regulators plays a key role. The kinases that mediate these phosphorylation events and the proteins within their signaling networks, however, are not known and their identification is the aim of this proposal. Aiming to identify the signaling pathways and key regulatory candidates during Plasmodium falciparum sexual development and consequently transmission, the overall goals of this proposal are: 1) Develop a novel tool to enrich early stage gametocytes from parasite culture using cell-type specific surface biotinylation and affinity purification. 2) Identify the signaling pathways that are used during the early stage of sexual development by near-system-wide quantitative phosphoproteome analysis comparing early stage gametocytes from Aim 1 with asexual parasite stages. 3) In a complementary approach, systematically test 10 kinase knockout lines for their ability to form gametocytes and gametes.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Malaria is a devastating disease caused by the apicomplexan parasite Plasmodium. Plasmodium falciparum represents the most severe and life-threatening form of human malaria, causing over 500,000 deaths per year. There is no efficient vaccine available and resistance to all currently used drugs reported. One of the major problems in eradicating human malaria is the transmission of the parasites sexual stages, the gametocytes. These are formed in the blood and develop through five different morphological stages in specific organs of the human host. When fully mature after 10-12 days, gametocytes are released into the blood stream to be transmitted by the mosquito. The molecular regulatory mechanisms important for the formation of gametocytes are poorly understood but there is evidence showing that phosphorylation of epigenetic regulators plays a key role. The kinases that mediate these phosphorylation events and the proteins within their signalling networks, however, are not known and their identification is the aim of this proposal. Specifically I will: 1) Develop a novel tool to enrich early stage gametocytes from parasite culture using cell-type specific surface biotinylation and affinity purification. Development of this novel technology will represent a significant enhancement in our ability to study early stage gametocytes, which are currently very hard to isolate from cell culture.2) Identify the signalling pathways that are used during the early stage of sexual development by near-system-wide quantitative phosphoproteome analysis comparing early stage gametocytes from Aim 1 with asexual parasite stages.3) In a complementary approach I will systematically test 10 kinase knockout lines available in the lab for their ability to form gametocytes and gametes. The function of the kinases identified will be verified by gene complementation.

Оригинален текст от CORDIS (на английски).

Участници

THE FRANCIS CRICK INSTITUTE LIMITED · LondonКоординаторОбединеното кралство
UNITED KINGDOM RESEARCH AND INNOVATION · SWINDONОбединеното кралство