H2020Индивидуална стипендия2015–2017

ECBStrainShift · Identification and characterization of the sex pheromone sensitive response gene(s) in the European Corn Borer males

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-10-15 → 2017-10-14
Финансиране от ЕС: 177 599 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Гените, които определят как мъжките европейски царевични молци реагират на различните пропорции от женски феромони, са обект на анализ. Разбирането им помага за по-ефективен мониторинг и управление на тези вредители в земеделието.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Identification and characterization of the sex pheromone sensitive response gene(s) in the European Corn Borer males

The genetic basis of olfaction is a topic of broad significance from fundamental and applied perspectives. The sense of smell is vital for most animals in finding food, identifying mates and avoiding predators. This study gave new insights on how moth pest species may evolve new pheromone preferences, which can cause race formation and speciation. Despite the importance of pheromone communication in reproductive isolation, its genetic basis is still unknown. By identifying the gene(s) responsible for variation in male response to female pheromone, in an important moth pest species, we give a first example on how response variation can cause strain differentiation. The European Corn Borer (ECB) pheromone strains are a unique example where sex pheromone differentiation precedes ecological differentiation. We investigated male responses in the two pheromone races of the ECB. Females of the E strain produce E11- and Z11-14:OAc in a 99:1 ratio, and females of the Z strain emit a 3:97 ratio of the same compounds. Males of each strain are attracted to the ratios emitted by the corresponding females. However, the gene(s) underlying the olfactory and behavioral shift in male response (resp) is still unknown. We aimed to characterize the genetic change underlying male response in this species, using a multidisciplinary approach, including classical chemical ecology, molecular biology, genetics and physiology. Thanks to this approach our work is of broad interest and use within the scientific society. Elucidating the genes underlying male olfactory responses in moths is also important from an applied perspective, because many moth species are important agricultural pests, and moth sex pheromones are an important tool in integrated pest management, used for monitoring, mating disruption and mass trapping. Identifying the gene(s) causing variation in male response can help monitoring populations for possible variations, and help to develop new pest control methods. Ultimately these methods target specifically one species and respect the environment. Objectives 1. Genetically isolate and characterize the genes that modulate the male pheromone preference. 2. Localize them in the olfactory tissues. 3. Functionally characterize them.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Night-active Lepidoptera are among the most speciose group of animals (~120.000 known species) and they possess a unique sexual communication system: females attract conspecific males by using a species-specific sex pheromone blend. Due to this fine-tuning, the development of novel pheromone systems is an evolutionary mystery: production of a new pheromone blend by females and preference for that blend by males must be established simultaneously, which is difficult to explain when the female blend and the male response are controlled by independently assorting genes. The pheromone strains of the European Corn Borer, Ostrinia nubilalis provide one of the best model system for a better understanding of pheromone (co)-evolution, because in these strains the females produce opposite proportions of E11- and Z11-tetradecenyl acetate, to which only strain-specific males are attracted. The genes responsible for the strain difference in female production are autosomally encoded and identified as fatty-acyl reductase while the gene(s) underlying male response has(have) been found to be sex-linked but have not been identified yet. For years they have been hypothesized to be olfactory receptors (ORs). However, we found with fine-scale mapping that the ORs map away from the Z-linked response locus and we showed two new candidate genes mapping at the male response region on the Z chromosome. The new candidates are involved in neurogenesis in other species and therefore are interesting for all olfaction and neurogenesis studies, including studies on humans. With our multidisciplinary experience, combining genetic engineering, neurophysiology and behaviour, we wish to identify the genetic change of the genes underlying male response in O. nubilalis and functionally characterize their involvement in olfaction. This approach has the potential to a) identify novel genetic mechanisms that underlie the evolution of pheromone communication and b) develop new pest management strategies

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITEIT VAN AMSTERDAM · AmsterdamКоординаторНидерландия