NETPAC · Microbial networks for PAC cycling in polluted soils
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2015-06-16 → 2018-07-13
- Финансиране от ЕС
- 239 191 €
- Участници
- 2
- Схема
- MSCA-IF-GF
Линиите свързват координатора с партньорите.
Накратко на български
Микробните общности в замърсени почви се анализират, за да се разбере как разграждат вредни вещества като полицикличните ароматни въглеводороди. Това помага за подобряване на методите за почистване на почвите и по-точна оценка на тяхната токсичност.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Microbial networks for PAC cycling in polluted soils
Polycyclic Aromatic Hydrocarbons (PAHs) are soil pollutants of special concern due to their recalcitrance and (geno)toxicity. Bioremediation, exploiting natural degrading capabilities of soil microorganisms, is a sustainable alternative for the restoration of those soils. However, this biotechnology is still not sufficiently effective, due to i) the limited degradation rates of PAHs; and ii) the presence of other toxicologically relevant polycyclic aromatic compounds (PACs), such as oxygenated PAHs (oxy-PAHs) and nitrogen heterocyclic PACs (N-PACs), whose fate and, in the case of oxy-PAHs, formation are normally overlooked. As a result, the success of bioremediation and its ecotoxicological assessment are often limited. NETPAC aims to identify the microbial communities and functions relevant for PAC biodegradation, and their adaptations to low bioavailability conditions, to further exploit them in novel and more reliable bioremediation approaches for PAH-impacted soils. Within NETPAC we applied state-of-the-art molecular microbial ecology and analytical chemistry tools in combination with stable isotope tracers to obtain a systems biology insight into the complex metabolic networks dealing with PAC-biodegradation and bioavailability in situ, integrating genomics, transcriptomics and metabolomics data. At this point, major contributions from NETPAC are: - To provide the first evidence of the direct implication of a bacterial metabolite of pyrene in increased soil genotoxicity after bioremediation. Formation of this heretofore unknown metabolite was associated to the activity of recently described groups of pyrene-degrading bacteria. - To develop a stable isotope assisted metabolomics approach to understand PAH-metabolism in situ. This method allowed reconstructing compound specific metabolic pathways in soils. - To identify an unprecedented abundance and diversity of N-PACs in PAH-contaminated soils by the application of nontarget high resolution analytical methods.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Polycyclic Aromatic Hydrocarbons (PAHs) are major soil pollutants causing special concern due to their high recalcitrance and (geno)toxicity. Despite the extensive knowledge gathered on microbial PAH degradation, current biorestoration technologies are still not sufficiently effective to decontaminate contaminated soils. One of the main characteristics that constrain PAH biodegradability in the environment is their low bioavailability for natural microbial communities, and the concomitant limited degradation rates. In addition, at PAH-polluted sites, other toxicologically relevant polycyclic aromatic compounds (PAC), such as oxygenated PAHs (oxy-PAHs) and nitrogen heterocyclic PAHs (N-PAHs), are generally present, their fate and, in the case of oxy-PAHs, formation being normally neglected. As a result, the success of bioremediation and its ecotoxicological assessment are often limited. NETPAC aims to identify the microbial communities and functions relevant for PAC biodegradation, and their adaptations to low bioavailability conditions, to further exploit them in novel and more sustainable approaches for biologically mediated restoration of PAH-impacted soils. Molecular microbial ecology and analytical chemistry methods in combination with stable isotope tracers will allow a systems biology insight into the complex microbial metabolic networks dealing with PAH-biodegradation and bioavailability in situ, by integrating genomics, transcriptomics and metabolomics data. Diagnostic tools will be developed and applied to monitor a lab-scale Green remediation approach based on enhanced natural attenuation, and to identify the natural microbial adaptations to promote the degradation of the expected low bioavailability residue. Understanding these processes will provide us with tools to assess biodegradation occurrence and, as a final outcome, predict the success of bioremediation thus reducing its uncertainties, one of the main drawbacks of this technology.
Оригинален текст от CORDIS (на английски).
Участници
- AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MadridКоординаторИспания
- THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL · Chapel HillСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз