H2020Индивидуална стипендия2015–2017

CHAT · Control of the Homeostasis of Actin through Time: actin homeostasis during embryonic development by means of single-molecule imaging and simulation in C. elegans

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-04-01 → 2017-03-31
Финансиране от ЕС: 185 076 €
Участници: 2
Схема: MSCA-IF-EF-RI

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Накратко на български

Динамиката на актинния скелет при ембрионите на червея C. elegans се анализира чрез наблюдение на единични молекули и симулации. Разбирането на тези процеси помага да се изяснят механизмите на клетъчното деление и как нарушенията в тях могат да доведат до рак.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Control of the Homeostasis of Actin through Time: actin homeostasis during embryonic development by means of single-molecule imaging and simulation in C. elegans

The actin cytoskeleton is a major determinant of the mechanical properties of embryonic cells and tissues. Spatial and temporal modulations of these properties play a major role in cellular transformation and important aspects of cancer such as cell division, polarity and migration. In embryonic cells, actomyosin forms a cortical reticulated gel that turns over rapidly, is weakly organized, and is composed of a variety of structures, with distinct architectures and dynamics. These structures are nucleated and elongated by different factors, and bind to specific sets of actin-binding proteins that modulate their dynamics. For example, in C. elegans, at least two types of cytoskeletal structures simultaneously coexist in the cell: short, branched actin filaments, nucleated by arp2/3, and long actin filaments/bundles nucleated by formins. Understanding how actin is distributed between these structures is critical to understand the biology and mechanics of the actin cytoskeleton and therefore its role in processes ranging from morphogenesis and cell division to endocytosis and polarization. We take advantage of high-resolution techniques to tease out the general rules and the critical regulatory elements that control actin dynamics. On the long term, this project may also improve our understanding of the general mechanisms that underlie the regulation of the actin cytoskeleton machinery, and how deregulations may be responsible for the onset of specific behaviors of the actin cytoskeleton that may eventually result in the development of cancer-like cellular behaviors.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In embryonic cells, actomyosin forms a cortical reticulated gel that turns over rapidly, is weakly organized, and is composed of a variety of structures, with distinct architectures and dynamics, which are nucleated and elongated by different factors, and bind to specific sets of actin-binding proteins that modulate their dynamics. Understanding how actin is distributed between these structures is critical to understand the biology and mechanics of the actin cytoskeleton and therefore its role in processes ranging from morphogenesis and cell division to endocytosis and polarization.In the proposed work, taking advantage of single-molecule techniques I developed during my previous post-doc, I will use the early C. elegans embryo as a model system to tease out the general rules and the critical regulatory elements that control actin homeostasis. I will follow three major directions: (1) analysis of actin dynamics in a steady-state system, the 1-cell stage embryo during maintenance phase, (2) analysis of the mechanisms underlying actin homeostasis in face of changes in the concentration landscape and biochemical properties of the players and (3) analysis of the modulation of actin dynamics and homeostasis during early embryonic development and between different cell types.I propose here a research program to expand our knowledge of how the structure of the actin meshwork is regulated and controls morphogenesis in embryonic cells, and in particular the mechanical properties of the cell. Importantly this program explores how modulations of the concentrations of actin-interacting proteins impinge on the dynamics of the actin cytoskeleton as a whole, and should improve our understanding of the general mechanisms that underlie the regulation of the actin cytoskeleton machinery, and how deregulations may be responsible for the onset of specific behaviors of the actin cytoskeleton that may eventually result in the development of cancer-like cellular behaviors.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE · ParisКоординаторФранция
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisФранция