H2020Индивидуална стипендия2015–2017

IMPAM · Development of a new immunoassay platform based on porous antibody microparticles

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-10-01 → 2017-09-30
Финансиране от ЕС: 159 461 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Нова платформа за диагностика използва порести микрочастици с антитела за откриване на вещества като токсини или маркери за рак. Тя позволява по-висока чувствителност на тестовете и намалява количеството скъпи реагенти, необходими за анализа.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Development of a new immunoassay platform based on porous antibody microparticles

The efficiency and at the same time price availability of in vitro tests systems for analyte determination are one of the main critical needs in medical and environment diagnostics, for example for the control of toxins, cancer markers, markers of inflammation, etc. The modern research directs on reaching these tasks. The traditional and widespread approach in the field of diagnostics (enzyme-linked immunosorbent assay, ELISA) has sensitivity limitations due to the use of planar supports for the detection of analytes. Besides that, the reduction of the amount of expensive reagents, such as antibodies, required to perform reliable testes, is crucial to make these tests readily available. In this project, we developed a new approach for biosensing based on porous antibody-contained microparticles that addresses these issues. The comparison of the new version of an immunoassay designed by us with traditional ELISA showed the following advantages of our system: 1) a higher sensitivity and 2) reducing the amounts of antibodies required to perform assays. Thus, the crucial tasks were solved. We believe that our new approach for the determination of analytes can expand the range of users, being useful in a wide diapason of analytical applications, and will be implemented in medical practice. Thus, the aims declared for the action were achieved.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Rapid and sensitive analyte determination in small volumes is one of the main tasks in modern diagnostics for the development of miniaturized in vitro test systems. This is critically important for both health and environmental diagnostics. The traditional approach (ELISA) is a time-consuming multistep process with sensitivity limitations due to usually used planar supports for detection. In this project we intend to develop a new approach for biosensing based on porous antibody microparticles. For this purpose, the combination of a new technique to assemble porous particles at mild conditions (host know-how) and highly sensitive ELISA-based detection system (applicant know-how) will be used. The porous particles will work as a platform with two crucial features: i) specific immunochemical reactions are realized on highly developed particle surface, ii) separation and detection in small volumes (micrometer dimensions). We believe that the proposed approach may open new avenues in future for effective diagnostics.

Оригинален текст от CORDIS (на английски).

Участници

FRAUNHOFER GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG EV · MunchenКоординаторГермания