H2020Индивидуална стипендия2015–2017

MASAM · New tools for key questions in plant development: using qualitative and quantitative proteomics for direct determination of the set of DNA and RNA-binding factors regulating single copy genes

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-11-01 → 2017-10-31
Финансиране от ЕС: 160 800 €
Участници: 1
Схема: MSCA-IF-EF-CAR

Линиите свързват координатора с партньорите.

Накратко на български

Факторите, които регулират работата на гена STM при растението Arabidopsis thaliana, се анализират чрез протеомика и масспектрометрия. Това помага да се разбере как се контролират стволовите клетки в растението, които формират всички негови надземни части.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

New tools for key questions in plant development: using qualitative and quantitative proteomics for direct determination of the set of DNA and RNA-binding factors regulating single copy genes

Plant development relies on the activity of meristems, a structure that contains stem cells that form plant organs. The shoot apical meristem (SAM) located at the growing tip generates all the aerial parts of plants through differentiation. To understand how the activity of the SAM is regulated it is then important to focus on the regulation of the activity of key genes acting in the SAM. SHOOT MERISTEMLESS (STM) from plant model Arabidopsis thaliana is known to be required for the maintenance of the activity of the shoot apical meristem. However the factors that bind regulatory regions of STM are largely unknown. The overall objectives of the project are to determine the factors that regulate the expression of STM. For this purpose we used a proteomic approach, using DNA-binding affinity coupled with mass spectrometry (DA-MS). We focused on the regulatory region F3 from STM promoter that was stated as important for STM regulation previously by the experienced researcher. Another goal in this project is to establish this procedure to be used in other regulatory regions of genes to study biologically relevant processes.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Plant development is based on the activity of meristems. The aerial part of plants is generated from the activity of the shoot apical meristem (SAM). These meristems determine the plant architecture and correct plant architecture have an immense impact in crop productivity. In the plant model Arabidopsis thaliana, a key gene for the correct functioning of the SAM is SHOOT MERISTEMLESS (STM). To study the function of this gene and to apply this knowledge to control plant architecture to generate valuable new plant varieties is of vital importance to determine how this gene is regulated throughout development. Gene regulation is controlled mainly by the action of transcription factors at transcriptional level and also by RNA-binding factors at post-transcriptional level. Then the goal of the project is to determine the set of transcription factors and RNA-binding factors that regulate STM throughout plant development. Current techniques for the determination of the set of factors regulating a gene give partial results, are time-consuming and require huge amounts of biological samples. Then, new tools have to be developed. The most promising ones involve the use of quantitative Proteomics with the latest advances of high-resolution Mass Spectrometry. We take advantage in the recognized expertise in this field of Proteomics by the host institution to develop these new techniques and put them available for the research community. In particular, the results and new discoveries obtained from this project are intended to represent a great progress in plant research.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE DE NAMUR · NamurКоординаторБелгия