H2020Индивидуална стипендия2015–2017

POTENTIATE · A Molecular Framework for Plant Cell Totipotency

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2015-06-01 → 2017-05-31
Финансиране от ЕС: 165 599 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Механизмите за превръщане на незрел полени в зародиши при рапса се анализират чрез специални маркери и секвениране. Това помага за по-бързото създаване на растения с определени наследствени признаци чрез селекция.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

A Molecular Framework for Plant Cell Totipotency

The plant kingdom is characterized by a high level of developmental plasticity, including the ability of plants to form embryos (totipotency) in the absence of fertilisation. Microspore embryogenesis (ME) is a form of totipotency in which immature (haploid) pollen is induced to form embryos in vitro. Haploid embryos can be converted to diploid homozygous (doubled-haploid, DH) plants in a single generation, placing ME in the centre of numerous breeding and trait discovery applications. ME was described more than 50 years ago, but a deep mechanistic understanding of ME and other forms of induced totipotency is lacking. It is, therefore, essential that we know the final fate of the different cell types in culture to be able to link these fates to specific signalling pathways and to understand their role in totipotency. Main objective in this project has been to isolate and characterize the different embryogenic cell types in Brassica napus microspore culture using a set of fluorescently-tagged gene reporters. The project has three main objectives: 1. Define and collect the different embryogenic cell types present in microspore culture using GFP-based reporters, time-lapse imaging, and cell sorting. 2. Define the transcriptional landscape of embryogenic cells using high throughput mRNA sequencing. 3. Determine the function of candidate microspore embryogenesis genes.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

This proposal aims to unravel to signalling pathways that underlie induced plant cell totipotency, by combining cell and molecular techniques to chart the changes that take place as differentiated pollen cells are induced to switch developmental pathways and form embryos in culture.Immature male gametophytes can be induced to change their developmental fate from pollen to haploid embryo development when exposed to stress treatments in vitro. This process (microspore embryogenesis) is widely exploited in plant breeding to generate homozygous (doubled-haploid) lines in a single generation, and also provides a unique system to understand plant totipotency and early cell fate decisions. In this proposal, I aim to understand the molecular basis for this switch in developmental pathways in Brassica napus. I will combine cell tracking, cell sorting of fluorescently tagged cells and high-throughput transcriptome sequencing to chart the changes that take place as immature pollen are converted into haploid embryos, and will also determine the function of a selection of genes in the model plant, arabidopsis. The project will advance my career by broadening my existing cell biology expertise, by providing training in a new field, molecular biology, and by allowing me to work with the plant model, arabidopsis. In addition, my network will be expanded to include a large group of internationally active scientists and seed companies. This novel combination of classical and state-of the-art skills, combined with the experience of working with top international groups and a plant breeding company, will place me in a solid position for a career in academia or industry.

Оригинален текст от CORDIS (на английски).

Участници

WAGENINGEN UNIVERSITY · WageningenКоординаторНидерландия