APTASENS · A new generation of AptaSensors

A new generation of AptaSensors

The project was focused on the design of different next generation libraries of biosensors that will yield ready-to-go analytical tools against any target in unprecedented speed and high affinity. Aptamers (DNA and peptides), a new generation of recognition elements were computationally selected and integrated into bioelectronics devices. The project objective to reduce by orders of magnitude the huge number of candidates to be tested using a combinatorial trial and error approach was carried out by rationalizing the strategy to design the aptamers. New virtual screening algorithms were developed for the generation of aptamers. The work was in line with the objectives of the project by providing small density array of electrodes modified with aptasensors having the sensitivity and reproducibility of the classical analytical methods. The conclusions of the action for WP1 were to provide databases with the main physicochemical properties of aptamers binding drugs, VOCs and Flaviviruses. In WP2 it was optimized the immobilization of the aptamers onto transducer surfaces along with demonstrating the utility of aptamers attached to dynamic and static nanoparticles and delivering the know-how for coupling functionalized aptamers with electrodes surfaces modified with gold nanoparticles. WP 3 tested different electrochemical techniques to have a label-free detection. Electrochemical impedance spectroscopy was widely studied because of the its unique property to explore the electrochemical surface without any label. Other electrochemical devices were considered like piezoelectric transducer suitable for gas sensors detection. WP 4 showed the analytical performances of the aptamers comparing them with classic analytical methods like optical and chromatographic methods. The main conclusions of the action were to produce small density arrays of aptasensors having the sensitivity and reproducibility of the classical analytical methods.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз