PACE · Paleogenetic extraction and analysis from challenging environments
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2016-06-01 → 2018-05-31
- Финансиране от ЕС
- 187 866 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Древната ДНК от човешки кости се извлича от трудни условия, като например горещи и влажни или сухи райони. Това помага за по-доброто разбиране на еволюцията и взаимодействието на хората в различни части на света.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Paleogenetic extraction and analysis from challenging environments
This project’s overarching aim is to study ancient DNA preservation in challenging environments, particularly from archaeological collections from warm and humid or warm and arid environmental contexts. In this way, we are looking to increase DNA yield from samples that normally do not contain a large amount of endogenous DNA (DNA specific to the individual sample). We chose to focus on a wide temporal span, from as early as the Neolithic (~5000 years before the present, yBP) to 800 yBP. Our research focuses on the human petrous bone as this has consistently shown to have a higher amount of endogenous DNA compared to other bones in the human skeleton. Our sampling approach consists of targeting the inner osseous canal, consisting of the cochlea and semi-circle canals. We use advanced DNA extraction and next-generation library sequencing methods to obtain our stated goals. This research is important for the ancient DNA community as it attempts to expand our understanding of how humans interacted and evolved in different regions of the world. Our overall objectives are to create more efficient sampling protocols to achieve our stated goals.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The field of ancient human DNA (aDNA) has rapidly undergone changes over the last decade. These changes have been spurred on through the use of high-throughput sequencing (HTS) technologies. These methodological advancements have increased our understanding of the human lineage to depths thought impossible only a short time ago. For example, aDNA research has shown how modern humans are related to our archaic ancestors, indicating significant admixture events throughout our evolutionary history with the Neanderthals and other now-extinct archaic species. However, to achieve these results, the ancient material has been exceptionally well preserved, owing to favorable, i.e. cold, environmental conditions. For many parts of the world, however, the environmental conditions remain a barrier to the extraction and analysis of aDNA. The nature of aDNA research is inherently interdisciplinary, whereby several branches of inquiry, such as archaeology or genetics, attempt to answer similar evolutionary questions from different perspectives. This proposal attempts to use advanced extraction and sampling methods for aDNA from challenging environments in order to broaden the scope of paleogenetics research. Although continental Europe has gained significantly from these advances, for other parts of the world, such as Southeast Asia and the Near East, this has been more difficult due to formidable preservation conditions. The goal of this proposal is to build on promising preliminary results that target a specific bone in the human skull in order to gain a better understanding surrounding the dynamics of aDNA preservation, while at the same time, gain insight into the evolutionary histories of peoples from warm and humid, or warm and arid, locations. Our proposal incorporates state-of-the-art HTS techniques, includes a comprehensive training program with international collaborators, and benefits from a two-way transfer of knowledge between host and researcher.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN · DublinКоординаторИрландия
Връзки
Данни: CORDIS, © Европейски съюз