H2020Индивидуална стипендия2017–2018

CCDSA · Control of cell division in Sulfolobus acidocaldarius

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-01-01 → 2018-12-31
Финансиране от ЕС: 195 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Процесът на делене на клетките при археята Sulfolobus acidocaldarius се анализира чрез изображения с висока резолюция и генетика. Това помага да се разбере произходът и работата на по-сложния клетъчен цикъл при еукариотите.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Control of cell division in Sulfolobus acidocaldarius

Eukaryotes evolved from a symbiotic merger of an archaeal host with a bacterial endosymbiont, prompting a dramatic and extensive reorganisation of cellular structure. Despite having diverged approximately 2 billion years ago, extant archaea and eukaryotes share core aspects of cell cycle control and conserved components of the cell division machinery. Comparative genomic analyses predict that the archaeal cell cycle could serve as a streamlined, minimal model for the far more elaborate eukaryotic one- yet little is known about how most archaea grow and divide. In proposing to use a combination of high-resolution imaging, genetics and quantitative cell biology to investigate the cell cycle of the model archaeon Sulfolobus acidocaldarius, we had two primary goals: to establish an archaeal model for the cell cycle, and to gain new insights into eukaryotic cell cycle control through an understanding of its origins.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Archaea and eukaryotes share conserved regulators such as ESCRTIII proteins that are involved in fundamental aspects of eukaryotic cell division, a finding that assumes particular importance in the context of widely accepted models for the evolution of eukaryotes from an ancestral archaeal host. Yet the mechanisms by which archaea regulate cell division- in the absence of nuclei, organelles and a rigid cell wall- are poorly understood. The research programme proposed here aims to fill this gap in our understanding by investigating the control and coordination of cell division in the model crenarchaeote Sulfolobus acidocaldarius. We will use super-resolution imaging in combination with cell sorting and electron cryotomography to characterize cell cycle progression in Sulfolobus at unprecedented resolution. We will leverage a novel in-house high-temperature live imaging platform and proteins labeled with thermostable GFP to dissect the molecular coupling between the key processes of chromosome segregation, membrane invagination, and abscission. Further, we will use forward genetics, whole-genome sequencing and comparative genomics to identify novel cell division regulators that are conserved in eukaryotes. We expect this detailed characterization of cell division in cells without organelles or a nucleus to have broad implications for our understanding of the origins of the more elaborate mechanisms found in extant eukaryotes. Moreover, in the future we expect the knowledge gained and interdisciplinary approaches developed here to be applicable for the study of Loki, the recently identified closest archaeal relative of eukaryotes, and for furthering the industrial utility of hyperthermophilic archaea.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITY COLLEGE LONDON · LondonКоординаторОбединеното кралство