H2020Индивидуална стипендия2016–2018

AlgDates · Dating Plastid Endosymbiosis and Diversification in Eukaryotic Algae

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-07-01 → 2018-06-30
Финансиране от ЕС: 173 076 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Еволюцията на водораслите и начинът, по който са придобили хлоропласти, се проследяват чрез генетични данни от видове с добре запазени окаменелости. Това помага да се разберат процесите на адаптация и развитие на водораслите в контекста на промените в климата.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Dating Plastid Endosymbiosis and Diversification in Eukaryotic Algae

During the eukaryote evolutionary history, the acquisition of plastids and the evolution of algae is one of the major transitions that has heavily shaped the earth and its biosphere as it is today. Still, few is known about the ancient endosymbiotic events, the evolution of these algal lineages and the correlation with the earth geological past conditions. One of the main problems, is that we lack molecular data for key extant species with good fossil record, which is fundamental to date the speciation events and help to resolve phylogenetic relationships between extant living species an reconstruct the evolution through the geological time scale. In the current situation of rapid climate change, we need to understand the adaptation and diversification of algae, which are of fundamental ecological importance. We addressed this issue by obtaining molecular data of calcareous algae with a high quality fossil record (Corallinales and Dasycladales) and including them in a plastid-wide phylogenomics study, including all known lineages of Cyanobacteria (the lineage that gave rise to the chloroplast in eukaryotes), the primary algae (Glaucophyta, Rhodophyta and Chloroplastida) and complex algae derived from secondary eukaryotic endosymbiotic events (Haptophyta, Ochrophyta, Cryptophyta, Euglenozoa and Chlorarachnophyta). The results include 2 newly sequenced algal genomes of calcareous red algae including its whole chloroplast genome, a state-of-the-art phylogenomic dataset including over 80 plastid markers (~20,000 amino acid positions) for a wide taxon sampling of more than 300 species, and 10 highly confident calibration points, and a timed tree with narrower time estimates than previous eukaryote-wise studies including diversification rates of each lineage and correlation with paleoclimatic data.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Eukaryotic algae evolutionary history is still fairly unknown. Thanks to state-of-the-art evolutionary models, next-gen sequencing and high computational capabilities, we are going to shed some light on the matter.Corallinales (red algae) and Dasycladales (green algae) are two extant living groups of calcareous algae with a rich fossil record (~140 and ~500 myr respectively). The lack of sufficient molecular data for these groups makes impossible to use such species in eukaryotic phylogeny and dating studies. This project has been planed to cover 2 objectives or evolutionary questions:The 1st objective aims 1) to generate de novo transcriptomic data for about 10 species corresponding to different Corallinales and Dasycladales lineages in order to perform phylogenomic analyses with 2 established datasets (one of 200 nuclear-coding plastid markers, and the other with 258 non-plastid genes) and the broadest possible eukaryotic taxonomic sampling; and 2) to perform molecular clock analyses using known fossil calibrations and hence provide robust divergence times for the whole eukaryotic tree but focusing on primary and secondary plastid endosymbiotic acquisitions. For both post- phylogenomic analyses (dating and divergence steps), already established collaborations will ensure proper method implementation.Using the results from the first objective, the 2nd one aims 1) to track history of plastid evolution (plastid-based dataset) comparing it with deep eukaryotic speciation events (non-plastid marker tree); and 2) to estimate diversification rates in distinct photosynthetic lineages in order to find if rate shifts correlate among them.AlgDates will allow us to establish the order, timing and correlation of events in such deep evolutionary transitions, but the knowledge acquired during this project will also provide information regarding reef formation and evolution, which can be useful when addressing or predicting calcareous ecosystem adaptations to climate change.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE PARIS-SACLAY · Gif-Sur-YvetteКоординаторФранция