H2020Индивидуална стипендия2017–2019

Exosomes · Regulated secretion and role of urinary nanovesicles

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-09-01 → 2019-08-31
Финансиране от ЕС: 200 195 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Уринарните нановезикули (екзозоми) се анализират, за да се види как съдържанието им се променя при бъбречни заболявания, диабет или високо кръвно налягане. Това помага за откриването на биомаркери, които да отразяват състоянието на бъбреците чрез анализ на урината.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Regulated secretion and role of urinary nanovesicles

Kidney disease, and its associated diabetes or high blood pressure, pose a great health risk for millions of people worldwide. The kidneys are part of the urinary tract that produces urine. Therefore, urine contains potential substances that can indicate the (patho)physiological conditions of the kidney. The purpose of this project is to examine the contents and role of the secreted urinary nanovesicles, in particular, exosomes, under different (patho)physiological conditions, with the hope of finding potential biomarkers from urinary exosomes. We have employed a transdisciplinary approach where multiple techniques including proteomics, bioinformatics and cell biology were used. Overall, we have revealed a proteome-scale positive correlation between urinary exosomes and kidney, indicating that urinary exosomes could reflect the physiological changes in the kidney to some extent. We also generated a comprehensive proteome database on urinary exosomes and kidney under different (patho)physiological conditions, facilitating targeted biomarker discovery.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Extracellular nanovesicles called “exosomes” are small membrane-bound vesicles that are secreted by a variety of cell types. Exosomes contain various molecular constituents of their cell of origin, incl. proteins and genetic materials. The exosome release pathway contributes towards protein secretion, antigen presentation and pathogen transfer and roles of exosomes in intercellular communications and transfer of materials have been studied in immunology, neurobiology, stem cell and tumor biology. Exosomes are also released into the urine, although their role in urine is unclear and provides the basis of this fellowship. To gain novel information about urinary exosomes, a transdisciplinary approach is required. I will use my current knowledge of qualitative and quantitative proteomics combined with extensive bioinformatics to study urinary and renal exosomes and their ‘cargo’ secreted under normal and pathophysiological conditions. I will combine this information with new ideas and techniques I will learn in my host laboratory to examine rates of exosome secretion and to assess whether exosomes have the capacity to inhibit the growth of bacteria. Methodologies in this fellowship include techniques which I have extensive experience of e.g. large scale proteomic profiling, and novel techniques that I will learn in the host lab to enhance my research portfolio and future career prospects, including cell biological and microbiological techniques and experience in collecting/using samples from patients. The scientific goals of the project are to investigate the underlying mechanisms for regulated exosome secretion and the role of exosomes in innate immunity. As exosomes are secreted from multiple cell types, this study will have major implications for a variety of biology disciplines. The fellowship will develop my career by providing new novel laboratory skills, it will give me greater freedom to independently run a research project and increase my int collaborations.

Оригинален текст от CORDIS (на английски).

Участници

AARHUS UNIVERSITET · Aarhus CКоординаторДания