H2020Индивидуална стипендия2016–2018

HeteroCancerInvasion · Effect of heterogeneity of cancer cells on collective invasion

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-05-01 → 2018-04-30
Финансиране от ЕС: 195 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Разнообразието между раковите клетки в един клъстер и начинът, по който те се движат заедно, определят как туморът нахлува в съседните тъкани. Разбирането на този процес помага да се разбере метастазирането, което е основна причина за смърт при пациентите.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Effect of heterogeneity of cancer cells on collective invasion

Cancer tissue is known to be composed of heterogeneous cancer cells and various stromal cells, and these cells are dynamically interacting each other. Although interaction between cancer cells and associated stromal cells in terms of tumour growth and resistance to anticancer drugs had been well studied, our understanding of association between heterogeneous cells in the context of invasion was very limited. Invasion of cancer cells into neighbouring tissue is a critical first step of metastasis, which is the leading cause of mortality among cancer patients. In the past decade, advanced imaging studies of both cultured cells and cells in living animals had revealed that cancer cells invade either as individually single cell or as well-organised adherent collectives. Extensive studies had provided substantial amount of insight into the molecular mechanism of how single cell moves; how to change their shape, activate the signalling pathway inside of the cells, and regulate cytoskeletal dynamics during migration/invasion. However, our understanding of collective invasion was less advanced. This partly reflected the likelihood that it is not a single invasive behaviour, but a combination of different behaviours. An additional complication was the well-documented heterogeneity of cancer cells even within a single tumour. This diversity may be genetic, epigenetic, related to ‘cancer stem cell’ hierarchies, or caused variation in local environmental cues. Therefore, to understand the process of collective cancer invasion one must consider possible heterogeneity between cancer cells in the cluster. This requires considering concepts such as relative migratory fitness, cell sorting, and cell competition. The objective of this project was to study collective cell migration involving heterogeneous populations of cells by using complementary experimental and computational models of collective cancer cell invasion.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Recent live cell imaging studies have demonstrated that cancer cells invade either as single cell or as cell group retaining cell-cell adhesion. In contrast to the well-documented single cell movement, little is known about collective cancer cell invasion. Given that a single tumour tissue contains various types of cancer cells and the collective movement of cancer cells is sum of the behavior of phenotypically different cells, we hypothesised that intratumour heterogeneity affect collective invasiveness. Careful dissection of the movement of diverse cancer cells is required to study the nature of collective invasion because different behaviour of heterogeneous cancer cells causes complication to understand the movement of whole cancer cell collective. In this study we will utilise and combine the single-cell resolution live cell imaging of 3D collective invasion assay with different types of cancer cell line generated by gene editing and mathematical modelling of invasion assay, which enable us to set up a lot of different possible starting configurations, to test our hypothesis.This project not only advance our current understanding of collective invasion of heterogeneous cancer cell population, but also will be beneficial to design better strategies to limit the invasion and metastasis of cancer.

Оригинален текст от CORDIS (на английски).

Участници

THE FRANCIS CRICK INSTITUTE LIMITED · LondonКоординаторОбединеното кралство