H2020Индивидуална стипендия2016–2018

CYgnaling · Probing the role of orphan Cytochrome P450 oxygenases in signaling compounds biosynthesis in plants by a comparative genomics and gene conservation approach

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-05-01 → 2018-04-30
Финансиране от ЕС: 185 076 €
Участници: 1
Схема: MSCA-IF-EF-RI

Линиите свързват координатора с партньорите.

Накратко на български

Ензимите от семейството на цитохром P450 при растението Arabidopsis thaliana се анализират, за да се открият непознати сигнализиращи съединения. По-доброто разбиране на тези процеси помага за подобряване на растежа, адаптацията и устойчивостта на културите в земеделието.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Probing the role of orphan Cytochrome P450 oxygenases in signaling compounds biosynthesis in plants by a comparative genomics and gene conservation approach

Plant are sessile organisms and thus permanently need to modulate their growth in order to optimize their exposure to light or access to nutrients. They also need to efficiently react to the pathogenic pressure. In agriculture in particular, plant growth, adaptation and resistance is a major challenge as they directly impact the yield and the quality of the output food. Better understanding the fine regulation leading to plant fitness is thus an important undertaking with huge implication in real-life economy. Plant growth regulators, such as phytohormones, are key factors for communication between parts of the plant, allowing a coordinated response of the whole plant to environmental challenges. These phytohormones are produced by specific biosynthetic pathways involving a cascade of enzymatic reactions. Currently, only a limited number of compounds have been characterized as plant growth regulators. They usually have drastic effects on plant development and have, for most of them, been known for many years. However, beside these players, recent works show that other signaling compounds with more subtle effects are also necessary for normal plant growth. The chemical natures of these compounds are yet unknown. In this project, we propose to study biosynthesis and action of overlooked plant hormones and signaling pathways using a reverse genetic approach in the model plant Arabidopsis thaliana (mouse-ear cress). In particular, we are focusing on genes coding for oxygenase enzymes belonging to the cytochrome P450 (P450) family. The P450 family plays a pivotal role in the known phytohormone biosynthesis or degradation, as all of their biosynthetic pathways, except one, require at least one P450 action to form a bioactive compound. Our main objectives in this project is (1) to select candidate genes and test their implication on plant development as a putative actor in phytohormone biosynthesis, (2) identify the chemical compounds that are being produced by the P450s of interest and more generally (3) to apprehend how the putative signaling pathways regulates plant growth.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

A limited number of molecules have been characterized as plant growth regulators or hormones, usually identified due to the strong effect that they exert on plant architecture or development. Most of the metabolic pathways leading to their biosynthesis and catabolism are now well characterized, and all but one involve oxygenases belonging to the family of cytochrome P450 (P450) oxygenases. The increasing set of sequenced plant genomes reveals the evolutionary history and complex phylogeny of the P450 superfamily. It shows that some P450 genes are specific to taxa or evolutionary branches, which correlates with the versatility of plant metabolomes when different species are compared. But comparative analysis of plant genomes also point to ancient genes, highly conserved and under strong purifying selection. Those are most often involved in the metabolism of molecules essential for plant development and with signaling function. Taking advantage of the increasing power of comparative genomics, we propose to identify P450s that share similar evolutionary characteristics with their counterparts in known hormone metabolism. We propose to perform a comprehensive functional analysis of a set of candidates, using the easiest plant model Arabidopsis thaliana, and to assess their role in the biosynthesis or degradation of signaling compounds. This includes establishing a detail map of candidates’ expressions to evaluate their place and time of action, and a phenotypic analysis of inactivation mutants and overexpressors to assess the consequences of gene misexpression on plant development. Based on gene expression and potential mutant phenotypes, we will select tissues to be analyzed for metabolic changes associated with gene mutation using high-definition analytical tools. Enzyme activity will be assessed with recombinant proteins. We are confident that this innovative multidisciplinary approach will be a powerful tool to identify new and overlooked plant signaling pathways.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisКоординаторФранция