NanoPorous DNA-array · Ultra-high density three dimentional DNA arrays for biosensing
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2016-09-01 → 2018-08-31
- Финансиране от ЕС
- 187 420 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Нанопористите ДНК-масиви се разработват чрез прецизно модифициране на мембрани за локализирано откриване на биомолекули. Тези сензори помагат за по-ранна диагностика на заболявания чрез откриване на минимално количество молекулярни маркери в телесните течности.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Ultra-high density three dimentional DNA arrays for biosensing
Biosensors are now among essential equipment and facilities in hospitals and health-care centers. Early diagnosis can save lives and therefore there is a great need for sensitive sensors that can detect minor amount of molecular markers in a body fluid. Nanosensors are among the most promising devices for such requirement. In the context of nanobiosensors it is important to achieve a method in which precise modification of the nanosensor is possible. This is a great challenge in the field of nanotechnology and it is not easy to produce nanomaterials with high precision. As at nanoscale the size of the material has a significant effect on its properties, it is necessary to precisely control the dimension of the nanomaterial for biosensing applications. In a series of work we aimed to introduce a method that can be used for precise manipulation of nanomterials which have potential applications is biosensing. We used helium ion microscope to reduce the pore size of a nanoporous membrane with a resolution of less than 1 nm. With the results of this successful study we made an overall objective to implement the new technique to combine nanofluidics and atomic force microscopy for localized detection of biomolecules.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Extra cellular, circulating nucleic acids – such as DNA or RNA – have been found as important biomarkers for identification and diagnosis of cancer and other diseases However, the detection of circulating nucleic acids is non-trivial because they often exist at very low concentrations in large volumes of body fluids. Although many attempts have been tried for the detection of these biomarkers in recent years, including various nanostructure based sensors, there is still no approach in sight that could reach the desired sensitivity. Hence, there is a great need for a sensor which can effectively detect the diagnostically relevant (low) concentrations of circulating nucleic acids with a high degree of selectivity and sensitivity. In this project we propose a new platform for detection of nucleic acids; the aim of this project is to achieve a multi-layered filtering device, which allows effective filtration, purification and detection of biomolecules from complex biofluids. The platform is based on implementation of ultra-high density 3D DNA arrays - which are made on a functionalized nanoporous substrate (nanoporous anodic aluminium oxide) – into a microfluidic device assay (so called FoRe assay, i.e combination of Forward and Reverse assays, which is consist of multiple filters, each capable of capturing specific target biomarkers from different sample). Pores are able capture the target molecules because locally the concentration of even a single target molecule in the pore is sufficient for binding and hybridization. The captured target molecules can then be detected by subsequent specific labeling using DNA-tagged gold nanoparticles (via hybridization), which will allow detection with single molecule sensitivity. If successful, the scalable system will be used as a point-of-care diagnostic platform in the future.
Оригинален текст от CORDIS (на английски).
Участници
- EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH · ZuerichКоординаторШвейцария
Връзки
Данни: CORDIS, © Европейски съюз