H2020Индивидуална стипендия2016–2018

PrinTendon · Development of a tendon/ligament substitute through bioprinting

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-09-15 → 2018-09-14
Финансиране от ЕС: 160 636 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

3D биопринтирането и магнитната стимулация се използват за създаване на изкуствени сухожилия и връзки от хидрогели и стволови клетки. Това помага за подобряване на възстановяването на тъканите, тъй като тялото трудно се справя самостоятелно с такива наранявания.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Development of a tendon/ligament substitute through bioprinting

Tendon/ligament injuries are a common clinical problem that can dramatically affect a patient’s quality of life. The native structure of tendons and ligaments makes them have limited ability to self-repair. Current approaches for tendons and ligaments substitutes include autografts, allografts and artificial prostheses although their mechanical limitations and/or the induced adverse immune responses have restricted their use, which have accelerated the development of tissue engineering strategies. However, to the date, no clinical long-standing acceptable tendons and ligaments substitute is available. The project’s overall objective was to explore the combination of 3D bioprinting and mechano-magnetic stimulation as cutting edge technologies to develop an innovative tendons and ligaments replacements that mimic their natural structure and function to induce cell and fibre alignment, which have been shown to induce tenogenic differentiation. Within this project, we investigated the use of different biomimetic approaches to establish the optimal biophysical and biochemical conditions to guide stem cells differentiation towards tendon-like cells. As well, in a second step, we developed cell-loaded injectable hydrogels (based on collagen and platelet lysate) loaded with magnetic particles that recreate the tendon niche and anisotropic architecture to produce 3D scaffolds for tendon regeneration using injectable and 3D printing techniques. Finally, we evaluated the in vitro differentiation of stem cells derived from adipose tissue within the scaffolds. In summary, this project has allowed to better recreate the tendon niche and to explore the use of 3D printing and magnetic particles to develop injectable hydrogels for 3D bioprinting, which will eventually help to improve the future strategies for tendon diseases treatment based on tissue engineering approaches. Furthermore, this project has allowed to the MSCA Fellow to acquire a very complete scientific profile with more professional independence and experience.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Tendon/ligament (T/L) injury is a common clinical problem that can dramatically affect a patient’s quality of life. The native structure of T/L makes them have limited ability to self-repair. Current approaches for T/L substitutes include autografts, allografts and artificial prostheses although their mechanical limitations and/or the induced adverse immune responses have restricted their use, which have accelerated the development of tissue engineering strategies. However, to the date, no clinical long-standing acceptable T/L substitute is available. For this reason, the project’s overall objective is developing an innovative T/L replacement that results in a fully regenerated living tissue, mimicking the natural structure and function and with long-term viability. The unexplored approach that we want take advantage of is through 3D bioprinting and mechano-magnetic stimulation. The use of bioprinting technologies will allow to replicate the tissue structure and together with mechano-magnetic stimulation will induce cell and fibre alignment, that have been shown to induce tenogenic differentiation. Hybrid collagen-silk fibroin scaffolds will be designed and bioprinted to satisfy the mechanical and biological properties needed for T/L substitutes. Mechanical stimulation will be achieved by the inclusion of magnetic nanoparticles in the scaffolds and the use of magnetic forces. Tissue-like constructs will be tested for its mechanical properties and its capacity to induce tenogenic differentiation of human adipose derived stem cells (hASCs), prior to an in vivo assay. This proposal combines the use of bioprinting and magnetic nanoparticles in a bright and new option to create T/L substitutes. Taking into account that hASCs can be easily harvested and expanded from autologous source and that 3D printers have high reproducibility and automation, this approach will assure an easy and fast translation to clinics to improve people’s live.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSIDADE DO MINHO · BragaКоординаторПортугалия