H2020Индивидуална стипендия2016–2018

CORA · Copper Acquisition

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-06-01 → 2018-05-31
Финансиране от ЕС: 178 157 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Археите, които окисляват амоняк, се нуждаят от мед, но механизмът, по който я усвояват от средата, остава неизвестен. Разбирането на този процес помага за подобряване на земеделските култури и пречистването на отпадни води.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Copper Acquisition

All organisms on Earth require certain types of trace metal co-factors like Fe, Mn and Cu for key enzymes in their metabolisms. Aerobic ammonia oxidation is one of the most important biological metabolic pathways of the nitrogen cycle on Earth and involves oxidation of ammonia to nitrite. Until 2005 it was believed that only bacteria contributed to this pathway. However, since 2005 it is now recognized that AOA are the main microorganisms controlling ammonia in terrestrial and aquatic systems. Ammonia oxidizing archaea (AOA) require copper (Cu) to carry out ammonia oxidation because it is a co-factor to many of the enzymes involved in this pathway. In contrast to bacteria, where various models of Cu acquisition and incorporation of Cu into enzymes exist, it is currently unknown how AOA acquire Cu in the environment. One hypothesis is that similar to bacteria, AOA secrete chalkophores—biological molecules that capture Cu and transport it back into the AOA cell. AOA might also take up Cu into the cell by an active transport mechanism or a cell pore that binds trace-metals. The key aim of project CORA was to determine how archaea take up Cu from the environment. This study was innovative because it is among the first to address this problem and will provide fundamental understanding to a key process that is of economic importance in agriculture and wastewater remediation. AOA provide crops with nitrogen for growth in soils and are used in remediating wastewater containing high levels of ammonia contamination. Results from this study will contribute to the fundamental understanding of Cu acquisition by archaea and will also be the first step to understanding an important factor that likely controls ammonia oxidation by AOA in terrestrial and aquatic environments.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

It has recently been discovered that archaea are promoting ammonia oxidation, an important process in nitrogen cycling. Ammonia oxidizing archaea (AOA) use Cu containing enzymes to oxidize ammonia to nitrate. Therefore, they have an elevated Cu requirement and thus the bioavailability of Cu to archaea may impact the nitrogen cycle. However, the molecular mechanism of Cu uptake by Archaea from terrestrial and aquatic environments remains unknown. Understanding the molecular mechanism of Cu uptake by these archaea will be an important step in understanding Cu acquisition by the Archaea. The first objective of this study will be to determine what types of proteins are potentially involved in Cu uptake under Cu limiting conditions. The hypothesis that low concentrations of Cu will result in expression of Cu acquisition genes will be tested using different strains of AOA originating from soil and hotspring environments and varying the availability of Cu to the AOA. The gene expression of AOA cultures as a function of Cu availability will be monitored using transcriptome illumina sequencing. The hypothesis that soil AOA will secrete extracellular reactive compounds to mobilize Cu as part of a Cu acquisition strategy will be tested. The presence of a strong copper binding ligands using a colorimetric assay and by an electrochemical method. The second objective of this study will be to use different strains of AOA in batch incubation experiments with different previously characterized Cu containing soils and minerals. The hypothesis that AOA isolated from soil environments will have high efficiency Cu uptake systems compared to AOA from the aquatic environment will be tested in batch incubations with AOA cell cultures and different Cu containing soils and minerals. This study will be innovative as this problem has not previously been addressed.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT WIEN · WienКоординаторАвстрия