H2020Индивидуална стипендия2016–2018

MuCellWall · Seed mucilage envelope as a new structural model in cell wall research

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-09-01 → 2018-08-31
Финансиране от ЕС: 171 461 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Слизистият слой около семената се изучава като специален вид клетъчна стена, изградена от полизахариди като пектини и целулоза. Визуализирането на тази структура с щадящи методи помага за по-доброто разбиране на архитектурата на клетъчната стена.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Seed mucilage envelope as a new structural model in cell wall research

In the present project the mucilage envelope was investigated as a specific anatomical structure at the cell level, where it represents a modified secondary cell wall possessing specific chemical composition, structure and properties. Mucilaginous cell wall is composed of three main groups of polysaccharides i.e. pectins, hemicelluloses and cellulose, where pectins constitute very often the main component. All of the polysaccharides are interacting to each other by means of diverse bonds (hydrogen, covalent or ion). The cell wall has been an important area for many different research disciplines however many studies concentrated mostly on (bio)chemical or genetic analysis. Our detailed knowledge about the cell wall architecture is still limited. The structural studies were mostly based on various invasive techniques such as enzymatic or chemical treatments, which can destroy and/or influence the cell wall organization and its individual components. The issue of this project was addressed to visualization of the spatial architecture of the mucilaginous cell wall in its almost native stage by using less invasive methods which should not destroy its structure and allow to detect and to observe the individual polysaccharides, their shape, size and distribution. The results coming from this project, including applied research techniques, can be significant for the broad society as well as for the industry.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The plant cell wall is dynamic, complexes structure composed of polysaccharides divided into fibrillar (cellulosic) fraction and extracellular matrix (hemicelluloses and pectins). All the components are arranged in a network structure characteristic of the cell wall. The proposed, general spatial models of the cell wall based mostly on various biochemical and/or microstructural analysis, which have usually been invasive by treatment with enzymes or chemicals, which can destroy the cell wall organization and allow to visualize a selected components. Despite of extensive research our knowledge about the cell wall is still incomplete and restricted mostly to the biochemical or genetic studies. The main aim of this project will be the determination of the cell wall components i.e. polysaccharides, glycoproteins, and the determination their spatial distribution in a special type of cell wall of the mucilage secreting cells (MSCs). The obtained results will allow creation a direct spatial cell wall model. The used material e.g. seeds of Arabidopsis thaliana mutants and wild type, Artemisia leucodens or Lepidium sativum will be an excellent and so far not examined study model due to characteristic loose spatial structure of the MSC cell wall. The use of modern techniques (in a quite new combinations) e.g. antibodies with attached gold particles and precise visualization in divers microscopes (e.g. SEM, LCSM) available at the host laboratory (Kiel University) will allow to overcome invasive procedures and let to detect the exactly location of the components. The realistic, representative cell wall spatial model will be created allowing for better understanding the different processes taking place within the cell wall. The project will be implemented at an interdisciplinary level opening a new possibilities for various cell wall studies. The project will give a new insight into the cell wall structural studies and lead to significant advances in this field.

Оригинален текст от CORDIS (на английски).

Участници

CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL · KielКоординаторГермания