H2020Индивидуална стипендия2016–2018

dsRNAEnvFate · Environmental fate of double stranded RNA (dsRNA) from RNA interference crop protection technology in agricultural systems: A systematic assessment of dsRNA hydrolysis, adsorption, and photolysis

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2016-07-01 → 2018-06-30
Финансиране от ЕС: 187 420 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Двойнонишковата РНК, използвана като биопестицид в ГМО култури за борба с вредители, се проследява в почвите и водите. Това помага да се разбере как тези вещества се разграждат и разпространяват, за да се оцени рискът им за околната среда.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Environmental fate of double stranded RNA (dsRNA) from RNA interference crop protection technology in agricultural systems: A systematic assessment of dsRNA hydrolysis, adsorption, and photolysis

Next-generation genetically modified (GM) crops seek to use RNA interference (RNAi) to protect crops through the expression of double-stranded RNA (dsRNA) biopesticides. Upon ingestion of the dsRNA biopesticide by a pest insect, the biosynthesis of targeted proteins by the insect is prevented, resulting in insect death. Some dsRNA biopesticides are rapidly approaching commercialization. However, limited information is available regarding the processes that govern the fate of dsRNA biopesticides after release from GM crops to surrounding environmental matrices (e.g., agricultural soils), resulting in uncertainty in exposure analysis required for environmental risk assessment of RNAi crops. To reduce these uncertainties, we have developed novel experimental approaches to evaluate the transport and transformation of dsRNA biopesticides in environmental matrices including soils, sediments and surface water bodies. Experiments investigated the impact of microbial and enzymatic degradation, abiotic transformation, and adsorption to particle surfaces on dsRNA fate in these environmental matrices. Overall, this work advances our understanding of the stability and distribution of dsRNA biopesticides in environmental matrices and informs exposure analysis required in environmental risk assessment models. The contribution will highlight the application of new experimental approaches to overcome methodological and conceptual challenges associated with studying the environmental fate of dsRNA biopesticides and other biomacromolecular pesticides produced by GM crops. By enabling research into the fate of these biomacromolecular pesticides, we can better evaluate potential environmental and health impacts associated with the use of next-generation GM crops protected by dsRNA and other biopesticides.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The use of RNA interference (RNAi) to combat pest insects is anticipated to become an integral component of crop protection technology in the US and Europe by 2020. Protection against pests is conferred via double stranded RNA (dsRNA) as the active ingredient that is either externally applied to the crop or internally expressed in genetically modified crops. Following ingestion by the pest, the dsRNA is cleaved to produce small interfering RNA (siRNA), which interfere with the translation of specific mRNA into proteins. While concern has been raised that the release of dsRNA into agricultural soils and adjacent water bodies may have detrimental ecological effects, risk assessment of RNAi crop protection technology has been impaired due to the lack of information on the fate and stability of dsRNA in the environment. The objective of the proposed project is to advance a detailed understanding of dsRNA fate in agricultural soils and adjacent water bodies. To this end, three environmental processes impacting dsRNA persistence will be studied on a mechanistic level: abiotic and enzymatic dsRNA hydrolysis, adsorption of dsRNA to mineral and organic particle surfaces, and direct and indirect photolysis of dsRNA. These processes will be studied using state-of-the art dsRNA quantification methods, experimental approaches, and analytical techniques employed in the environmental sciences, material sciences, and molecular biology. The project aims at unraveling the mechanisms and main reaction pathways that control dsRNA hydrolysis, adsorption and photolysis. Through the generation of a fundamental understanding of these processes, the proposed project will lay the foundation to predict the fate and stability of dsRNA in agricultural systems. As such, the results of this proposal will allow regulatory agencies to develop dsRNA exposure scenarios in agricultural systems and thus help advance risk assessment of RNAi crop protection technology prior to its implementation.

Оригинален текст от CORDIS (на английски).

Участници

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH · ZuerichКоординаторШвейцария