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H2020Докторантска мрежа2017–2021

ES-Cat · Directed Protein Evolution for Synthetic Biology and Biocatalysis

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период
2017-03-01 → 2021-11-30
Финансиране от ЕС
3 827 031 €
Участници
19
Схема
MSCA-ITN-ETN

Линиите свързват координатора с партньорите.

Накратко на български

Ензимите се модифицират чрез насочена еволюция, за да ускоряват химични реакции, които в природата не се случват. Това позволява създаването на по-ефективни биокатализатори за медицината и биотехнологиите, които работят при умерени условия без токсични вещества.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Directed Protein Evolution for Synthetic Biology and Biocatalysis

The ES-Cat (Evolution, Synthetic Biology, and Biocatalysis) Research Consortium aims to use directed evolution to reproduce and improve upon the remarkable biological catalysts (enzymes) found in nature. Enzymes are largely selective, efficient, and ‘green’; chemical reactions that may otherwise require high temperatures or pressures, harsh solvents, or toxic metal ions can be catalysed by enzymes in comparatively mild conditions. The exploitation of enzymes is limited, however, by the immense complexity of these biological molecules. The process of Darwinian evolution has shaped naturally occurring enzymes to fill their requirements in nature, so if we wish to harness the catalytic power of enzymes for non-natural reactions or reactions on substrates of our choosing, we must engineer them to suit our needs. The ES-Cat Consortium, therefore, is designed to share and combine complementary approaches to enzyme engineering, and to empower Early Stage Researchers (ESRs) with the tools required to generate efficient biocatalysts for application in biomedicine, biotechnology, and cell biology. The objectives of the Action are broadly: • to involve ESRs in design and application of innovative strategies for industrial protein engineering that integrate directed evolution, powerful computational methods, and miniaturized high-throughput experimentation • to engage ESRs in collaborative projects with industrial partners interested in robust proteins for use in applied biocatalysis, or medicine. • to train ESRs as creative researchers able to develop and apply novel methodologies in tailoring enzymes and other proteins for industrial application, by guidance and frequent contacts with PIs from the network, and through participation in scientific events. • to train ESRS in awareness of societal context of molecular biotechnology and of the industrial opportunities and challenges, as well as to equip them with the ambition and skills required for a successful career in industries contributing to the Europe’s competitive bioeconomy • to disseminate the vision, activities and results of the ES-Cat network to the outside world by workshops and symposia open to other EU project participants and network members, and their home institutes. In addition to making the case for the use of directed evolution in protein engineering, we will endeavour to raise citizen awareness by engaging with the public via open days, MC ambassadorships and social media.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

ES-Cat will use directed evolution as a tool to reproduce Nature's remarkable ability to generate molecular machines - in particular enzymes – that perform at levels near perfection. Instead of seeing rational and combinatorial approaches as alternatives, we combine them in this network to achieve a ‘smarter’ and more efficient exploration of proteinsequence space. By harnessing the forces of Darwinian evolution and design in the laboratory we want to (i) screen large and diverse libraries for proteins with improved and useful functions, (ii) optimize existing proteins for applications in medicine or biotechnology and (iii) provide a better understanding of how existing enzymes evolved and howenzyme mechanisms can be manipulated. This Network brings together leading academic and industrial groups with diverse and complementary skills. The range of methodologies represented in ES-Cat allows an integrated approach combining in silico structural and sequence analysis with experimental high-throughput screening selection methods (phage-, ribozyme and SNAP display, robotic liquid handling, lab-on-a-chip/microfluidics) with subsequent systematic kinetic and biophysicalanalysis. This integration of methods and disciplines will improve the likelihood of success of directed evolution campaigns, shorten biocatalyst development times, and make protein engineering applicable to a wider range of industrial targets. It will also train the next generation of creative researchers ready to fill roles in tailoring enzymes and other proteins for industrial application in synthetic biology efforts to move towards a bio-based economy, rivaling advances that are being made in the US and allowing the EU economy to harvest its evident socio-economic benefits.

Оригинален текст от CORDIS (на английски).

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Данни: CORDIS, © Европейски съюз