H2020Индивидуална стипендия2017–2019

DENOCS · The Double Edged Role of Nitric Oxide and Hydrogen Peroxide in a Coral Symbiosis

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-06-01 → 2019-07-01
Финансиране от ЕС: 212 195 €
Участници: 1
Схема: MSCA-IF-EF-CAR

Линиите свързват координатора с партньорите.

Накратко на български

Ролята на азотния оксид и водородния пероксид при разпада на симбиозата между коралите и микроалгите се анализира чрез нови методи за измерване. Това помага да се разберат механизмите, които водят до избелване и смърт на кораловите рифове при повишаване на температурата.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

The Double Edged Role of Nitric Oxide and Hydrogen Peroxide in a Coral Symbiosis

Climate changerelated rising sea surface temperatures challenge the integrity of the most diverse marine ecosystem – the coral reefs. Recent years marked the worse global coral bleaching event on record. Corals depend on a symbiotic relationship with microalgae (genus Symbiodinium) that they harbour in their tissue as their primary food source. Coral bleaching leads to a breakdown of this symbiosis and loss of algal cells, eventually leading to coral death if the symbiosis is not re-established. It is known that oxidative stress and production of reactive chemical species such as NO and H2O2 are involved in the breakdown of coral symbioses. Regulation and mechanisms affecting the dynamics of these reactive species in corals present a significant knowledge gap.This project employed novel microanalytical measuring techniques for NO and H2O2 quantification that enabled detailed studies of a coral stress response in terms of i) the cellular mechanisms , ii) the source of NO iii) direct quantitative measures of H2O2 of intact coral symbioses. Following research questions lead the project: (1) How can we optimize the assessment of NO and H2O2 in an intact coral symbiosis to study their dynamics and interaction on a quantitative and comparable basis? (2) Does carbon fixation in Symbiodinium and therefore the coral host’s organic carbon supply, decrease under nitrosative stress conditions thus exacerbating a stress response by starving the coral host leading to coral bleaching? (3) What are the production hotspots of key cellular compounds involved in an oxidative stress response within the coral tissue initiating coral bleaching?

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Global climate changes affect coral reefs worldwide and these valuable marine biodiversity hotspots are increasingly experiencing a symbiotic dysfunction known as coral bleaching, where the global bleaching events in 2016 are the most significant to date. The underlying physiological mechanisms inducing the bleaching response remain unclear. It is known that cellular oxidative stress and reactive species such as nitric oxide (NO) and hydrogen peroxide (H2O2) are involved, but the sources and sinks of these compounds, their interplay and spatio-temporal dynamics have not been investigated in corals, partly due to lack of suitable experimental tools. The DENOCS projects will apply a suite of novel quantitative assessment techniques to investigate the dynamics and impact sites of NO and H2O2 in intact corals, coral tissue culture, and isolated photosymbionts when subjected to experimental treatments mimicking global change-induced environmental stress scenarios. This will encompass expert training of Dr. Schrameyer in the use of novel microsensors for NO and H2O2 in combination with advanced bioimaging of the oxidative and nitrosative stress response in corals, variable chlorophyll fluorescence imaging and cellular 14C-fixation assays to assess symbiont photosynthesis. Prospective outcomes of DENOCS include a better understanding of oxidative and nitrosative stress responses in corals, quantification of threshold concentrations and impact sites of reactive oxygen and nitrogen species, and how these are involved in the coral bleaching response. The project DENOCS will promote EU’s research and innovation excellence through its interdisciplinary measurement approach and international collaboration, and will enable the restart of a promising career of Dr. Schrameyer after a maternity break.

Оригинален текст от CORDIS (на английски).

Участници

KOBENHAVNS UNIVERSITET · KOBENHAVNКоординаторДания