EpiMethFly · Study of the role of m6A RNA methylation in the nervous system of Drosophila melanogaster: an in-vivo model to dissect the impact of epitranscriptome reprogramming in physiology and cancer
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2017-09-19 → 2020-04-18
- Финансиране от ЕС
- 180 277 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Метилирането на РНК (m6A) се изучава чрез плодови мухи, за да се разбере как влияе върху развитието и връзките в нервната система. Тези знания помагат за разбирането на механизмите при рак на мозъка и други заболявания, което е важно за бъдещи терапии.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Study of the role of m6A RNA methylation in the nervous system of Drosophila melanogaster: an in-vivo model to dissect the impact of epitranscriptome reprogramming in physiology and cancer
The project is focused on understanding the role of RNA modifications and, in particular, one form of RNA methylation (m6A) during normal and aberrant development of the nervous system using a powerful genetic model, such as the fruit fly Drosophila melanogaster. The study of m6A is important for the society because, due to its central biological role, m6A might represent an important target to be exploited for the treatment of brain cancer and other pathologies of the nervous system. However, to do so we need to get a clear understanding of what are m6A targets and the processes that it regulates that is currently missing. The project wanted to address this problem: to give a better and more detailed insight into how m6A affects the biology of the nervous system and the molecular mechanisms underlying this function. More precisely, the project had two main objectives: the first one was to clarify the consequences of perturbing RNA methylation during Central nervous system development in terms of brain connectivity and assembly. The second overall objective was to study if perturbation of m6A plays a role in brain cancer (glioblastoma multiforme GBM) development to get a better insight into how m6A could be exploited for brain cancer therapy.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The identification of reversible m6A RNA modification strongly points towards the existence of a novel level of complexity of gene expression regulation. Recent studies have suggested that m6A might be involved in several biological processes and that dysregulation of this modification may contributes to neuro-developmental abnormalities and cancer. The aim of the described research is to clarify the role of m6A RNA modification in the nervous system in physiological conditions -during brain development - and to discover its contribution to pathological conditions - Glioblastoma. To achieve this goal I propose to use Drosophila melanogaster to study : Aim 1 the impact of perturbing different members of the m6A machinery on brain morphology, progenitors differentiation and neuronal axon growth and guidance; Aim2 the impact of modulating the levels of m6A RNA modification in a Glioblastoma model and study the effect of this perturbation on tumor growth and progression. The results obtained will be of fundamental importance for the development of the field of epitranscriptomics by shedding light on the biological outcome of m6A modification in-vivo. In particular, Drosophila might become an efficient model to screen for novel members of the m6A machinery and to clarify how m6A is read and translated into a biological response. Finally, the data obtained by the analysis of the Glioblastoma model, will strongly contribute to the understanding of the link between RNA methylation and tumor development and aggressiveness.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITA DEGLI STUDI DI TRENTO · TrentoКоординаторИталия
Връзки
Данни: CORDIS, © Европейски съюз