H2020Индивидуална стипендия2017–2019

AEGILWHEAT · Widening gene pool of bread wheat by hybridization with Aegilops biuncialis supported by advanced genetic and chromosome genomic approaches

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-09-01 → 2019-08-31
Финансиране от ЕС: 154 721 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Генетичните особености на дивата пшеница Aegilops biuncialis се анализират, за да се пренесат полезни гени за добив и устойчивост в културната пшеница. Това помага за създаването на сорта, който да издържа на стресови условия и да осигури храна за наражаващото се население.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Widening gene pool of bread wheat by hybridization with Aegilops biuncialis supported by advanced genetic and chromosome genomic approaches

Feeding the 9 billion human population in 2050 will require high yielding, stress tolerant wheat cultivars with good nutritional quality to produce enough food by sustainable and cost effective means with reduced chemical load of the environment. Breeding of improved wheat varieties could be accelerated by the use of genes which are absent in extant gene pool of hexaploid wheat. The wheat wild relative Aegilops species represent a rich source of genes for important traits including grain yield, quality and tolerance to abiotic and biotic stress, which can be utilized by interspecific hybridization. To date, the application of wild genetic diversity in breeding programs has been hampered by low throughput of methods used to select introgression lines and the lack of knowledge on the genomes of wild relatives. The objectives of multidisciplinary AEGILWHEAT project were to (1) isolate individual chromosomes 1Ub, 3Ub, 3Mb and 7Mb from Aegilops biuncialis and (2) identify their gene content and produce polymorphic markers (SNPs, INDELs) by next-generation sequencing and bioinformatic analyses. The genes and new markers will be integrated into (3) a new high resolution genetic linkage map of Ae. biuncialis. The marker-assisted selection system will be used for the (4) high throughput selection of wheat-Ae. biuncialis introgression lines.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The widening of gene pool of bread wheat (Triticum aestivum) by employing its wild relatives as donors of new alleles and genes will ensure the ability of wheat to sustain yield quality and quantity under adverse environmental conditions. Species from genus Aegilops represent a rich source of agronomically important traits, such as leaf rust resistance, grain nutritional quality and abiotic stress tolerance. To date, the utilization of wild genetic diversity has been hampered by low throughput of methods used to select introgression lines and the lack of knowledge on the genomes of wild relatives. The multidisciplinary AEGILWHEAT project integrates the applicant’s expertise in interspecific hybridization and cytomolecular genome analysis of Aegilops species with the state of art expertise in flow-cytometric sorting and genomic analysis of plant mitotic chromosomes in the Centre of Plant structural and Functional genomics (IEB, Olomouc, CZ). The aims of this project are to (1) isolate individual U and M chromosomes from Aegilops biuncialis and (2) identify their gene content and produce polymorphic markers (SNPs, INDELs) by next-generation sequencing and bioinformatic analyses. The genes and new markers will be integrated into (3) a new high resolution genetic linkage map of Ae. biuncialis. The marker-assisted selection system based on the ordered markers will be used for the (4) high throughput selection of wheat-Ae. biuncialis introgression lines. The training through the research will extend the expertise of the applicant by new skills in molecular genetics, genomics and bioinformatics so that he can apply innovative genomics approaches to speed up his wheat pre-breeding programmes in Hungary. The project will deliver valuable information to geneticists and breeders on chromosome structure and genome evolution within the tribe Triticeae and provide molecular tools to support cost-effective transfer of wild alleles into wheat.

Оригинален текст от CORDIS (на английски).

Участници

USTAV EXPERIMENTALNI BOTANIKY AV CR · PrahaКоординаторЧехия