H2020Индивидуална стипендия2018–2020

AntibioPICs · Enzyme-Degradable Polyion-Complex (PIC) Particles for the Treatment and Detection of Pseudomonas aeruginosa

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-03-12 → 2020-05-31
Финансиране от ЕС: 195 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Наночастици, които освобождават антибиотици или багрила само при наличие на бактерията Pseudomonas aeruginosa, се тестват за лечение и откриване на инфекции. Това помага за по-ефективното използване на наличните лекарства и ранното разпознаване на бактерии, устойчиви на множество антибиотици.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Enzyme-Degradable Polyion-Complex (PIC) Particles for the Treatment and Detection of Pseudomonas aeruginosa

In this project we had developed (nano)particles that can selectively release relevant antibiotic only if a pathogenic bacterial caused infections. Two exemplars had been explored: 1. Targeted delivery of polymyxins, antibiotic peptides currently used in the clinic as last resort antimicrobials. 2. Targeted delivery of imaging agents, to label microbial membranes in the infection site. the importance of our research comes from the fact that it addressed current challenges in antimicrobial research and in the development of new antibiotic targeting the multidrug resistance bacteria called superbugs. in particular: 1) the development of better methods to use currently available antibiotics. 2) the early detection of bacterial infections. The project objectives were: 1. Synthesis of enzyme Degradable Polymer to facilitate antibiotic encapsulation in nanoparticles called PIC particles. 2. Preparation of enzyme Degradable Polymer to facilitate FM® 1-43 Dye encapsulation in nanopartiCharacterisation of PIC particle stability and enzyme-degradation kinetics and selectivity 3. In-vitro evaluation of 1) antimicrobial activity of the prepared nanoparticles against pathogenic bacteria called P. aeruginosa and 2) ability of the FM-containing particles to stain and report the infection caused by this bacteria P. aeruginosa with colour change to naked eye.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Here, we will develop new enzyme-degradable polymers for the preparation of polyion complex (PIC) particles based of antimicrobial molecules with low charge densities. The main goals are:1. Synthesis of enzyme-responsive polymers, with a) enough charge density to facilitate PIC particle formation, and b) degradable by Pseudolysin, a protease secreted by opportunistic bacteria Pseudomonas aeruginosa;2. Preparation of Pseudolysin-Degradable PIC Particles, from these enzyme responsive polymers and 1) Polymyxin B (PolB), an antimicrobial peptide with only 5 cationic charges; or 2) FM® 1-43 Dye, a cationic membrane dye that stains gram-negative bacteria;3. Characterisation of PIC particle stability and enzyme-degradation kinetics and selectivity, and4. In-vitro evaluation of 1) the antimicrobial activity against P. aeruginosa of PolB-containing particles and 2) the ability of FM-containing particles to stain P. aeruginosa.Completion of these goals will allow us to demonstrate that:a) Polymers with high charge densities can be prepared based on short peptides; thatb) stable PIC particles can be prepared from relevant small molecules; despite the challenges posed by their low charge density; thatc) the stability and release profile of these biologically active molecules can be tuned as a function of polymer composition and particle formulation; and thatd) PIC particles are promising vectors for the delivery of antimicrobial molecules.The main scientific challenge lies in the development of the proposed materials, but the molecules to be delivered have been selected because of their relevance to antimicrobial resistance, one of the research priorities of the European Commission. Each exemplar will contribute to address complementary problems: 1) the development of better methods to use currently available antibiotics (PolB) and 2) the early detection of pathogenic strains (FM).

Оригинален текст от CORDIS (на английски).

Участници

THE UNIVERSITY OF BIRMINGHAM · BirminghamКоординаторОбединеното кралство