ColourFish · Zebrafish colour vision: a functional approach to studying outer retinal wiring strategies
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2017-05-01 → 2019-04-30
- Финансиране от ЕС
- 195 455 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Златистите рибки се използват, за да се проследи как сигналите от различните цветови рецептори се комбинират в ретината. Разбирането на тези процеси помага при разработването на генна и стволова терапия за хора с цветна слепота.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Zebrafish colour vision: a functional approach to studying outer retinal wiring strategies
Vertebrate colour vision starts with the use of spectrally distinct cone-photoreceptors. Loss of one or more cone types leads to partial or complete colour blindness. To cure colour blindness, gene and stem cell therapy methods have been developed as a means to introduce or replace the lost cone types. The next essential step towards clinical application of these methods is to understand how the downstream retinal circuits of cone-photoreceptors process chromatic information. In this project, we studied the chromatic information processing at the first synaptic layer of the visual system. Using highly visual model animal with robust colour vision, zebrafish, and by harnessing it’s genetic accessibility and transparent larvae, we achieved to record cone functions in live animal for the first time. With this tool, we studied how cone signals are combined and modulated to compute contrasts in wavelength in the retina’s outer plexiform layer. Further, we investigated how loss of one cone type impact the chromatic information processing in the retina.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Colour vision is critical animals to navigate their environment. Here, two or more spectrally distinct cone-photoreceptor types are needed in the retina which must be postsynaptically combined to extract contrasts in wavelength. Bipolar cells make selective connections with distinct cone types at the first synaptic layer of the retina and cone-signal integration by bipolar cells lays the foundation for vertebrate colour vision. However, how bipolar cells form connections with selective cone types and achieve functional integration of these inputs to extract chromatic signals during development remains poorly understood. We will focus on zebrafish that has robust colour vision and study the roles of cone inputs and composition in establishing bipolar cell chromatic wiring and function. We will use a multidisciplinary approach spanning genetic methods, anatomical analysis, physiological recordings and statistical modelling. Drawing on existing genetic tools and marker lines established by the applicant, we will focus on two genetically targeted bipolar cell types: one that is cone-selective and one that is cone-unselective. First, we will use in vivo time-lapse imaging to study how these bipolar cells formation dendritic contacts with cones during development. Next, we will use two-photon functional imaging of light-driven synaptic release from cones as well as both dendritic and axonal imaging in bipolar cells to study how chromatically distinct signals are functionally integrated. Finally, using further lines already established by the applicant, we will genetically silence or ablate individual cone types to study how functional integration and wiring formation are regulated by the activity or availability of cones. These studies will gain insight into the strategies to encode chromatic information in parallel pathways of the retina and thus advance our understanding in the role of colour vision circuits in driving animal behaviour.
Оригинален текст от CORDIS (на английски).
Участници
- THE UNIVERSITY OF SUSSEX · BrightonКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз