H2020Индивидуална стипендия2018–2020

DNCSS · Decoding neural circuits controlling sleep drive and sedation

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-09-01 → 2020-08-31
Финансиране от ЕС: 183 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Невронните вериги в мозъка контролират съня и действието на успокоителните лекарства. Разбирането на тези механизми помага да се разбере връзката между съня и неврологични заболявания като болестите на Алцхаймер и Паркинсон, което може да подобри създаването на по-безопасни медикаменти.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Decoding neural circuits controlling sleep drive and sedation

Sleep is a universal behaviour across the animal kingdom and humans spend on average one-third of their lives asleep. Surprisingly, the full picture of how sleep is regulated and why we need sleep is still yet to be completed. In mammals, sleep consists of two phases: rapid eye movement (REM) sleep and non-rapid eye movement (NREM) sleep. Both REM and NREM sleep are chronologically and homeostatically regulated by several brain regions. Previous studies have established the association between sleep disturbances and almost all the neurological disorders, including autism, Alzheimer’s disease and Parkinson’s disease. In addition, sleep has also been compared to sedation, a state sharing significant behavioural similarities with sleep, such as reduced movements, enhanced slow-wave activity and lowered body temperature. Whether sedatives work through a common mechanism of hijacking the sleep-regulating circuits still remains elusive. This project aimed to identify novel neuronal circuits controlling sleep and sedation. Discoveries of this project would significantly advance our understanding of the fundamental mechanisms of sleep/sedation and provide novel insights into how sleep is linked with various neurological disorders. It would also offer potential therapeutic strategies and contribute to designing more efficient and safer medications. The main scientific goal was broken down into the following three objectives: - To evaluate alterations of excitatory/inhibitory input onto defined LPO neurons during prolonged wakefulness and sedation. - To identify the origin of inputs onto the defined LPO neurons - To determine the effects of altered connectivity involving active LPO neurons on sleep and sedation status in vivo.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Sleep and anaesthesia are both commonplace states that involve reversible loss of consciousness. However, the precise regulatory mechanisms underlying both still remain elusive, particularly at the circuitry level. Previous studies suggest that sleep is homeostatically regulated and it has been proposed that the interaction between circadian rhythm and intrinsic sleep drive determines sleep status. Although much efforts have been made, the sleep field still lacks clarity regarding the nature of the “sleep drive” as well as how it modulates sleep homeostasis. My host lab recently revealed that neuronal ensembles in the PO area, particularly in the lateral PO (LPO) area, a small region at the base of the brain that contains a mixture of sleep-active, wake-active, and temperature-sensitive neurons, are selectively activated during recovery sleep and drug-induced sedation. These results indicate that the LPO neurons are able to sense the sleep drive. In this proposal my goal is to identify LPO associated functional connectivity encoding sleep drive and sedation. By combining TetTagging functional ensembles of neurons with in vitro and in vivo optogenetics, electrophysiology and imaging techniques in mouse, I aim to 1) evaluate alterations of excitatory/inhibitory inputs onto the defined LPO neurons during prolonged wakefulness and sedation; 2) identify the origin of inputs onto the defined LPO neurons; 3) tdetermine the effects of altered connectivity involving active LPO neurons on sleep and sedation status in vivo. The results of the proposed research will provide novel insights into the regulatory mechanisms underlying sedation and the homeostatic drive of sleep.

Оригинален текст от CORDIS (на английски).

Участници

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE · LondonКоординаторОбединеното кралство