H2020Индивидуална стипендия2017–2019

TRAIN SPOTTING · Mapping the Intraflagellar Transport - A High-resolution Study of Intraflagellar Transport Trains in Chlamydomonas Cells

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-08-01 → 2019-09-30
Финансиране от ЕС: 171 461 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Протеиновите комплекси в камшиците на клетките Chlamydomonas се анализират чрез високоразрешаваща микроскопия. Това помага за разбирането на начина, по който се сглобяват и функционират тези структури, които позволяват на клетките да се движат.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Mapping the Intraflagellar Transport - A High-resolution Study of Intraflagellar Transport Trains in Chlamydomonas Cells

I spent the whole project period at MPI-CBG Dresden by an intensive work and tight collaboration with the project host, Gaia Pigino, and her group. Even though the project objectives have slightly changed in comparison to the project proposal, we were able to collect results for two full original research papers which are now in preparation. Objectives of the project TRAINSPOTTING were setup both for my career development (WP1 and WP2) and research (WP3-5). Even though the research work packages have partially changed, we substituted them with objectives which were using identical methodological setup and focused on the structural and functional analysis of a difference ciliary component than IFT. The new protein complex investigated is also a fundamental constituent of all motile cilia/flagella and is required for the assembly of functional cilia/flagella. Therefore, the newly identified objectives had similarly significant research impact to the one proposed in the original application. These objectives were fully addressed. I was able to propose independently the scientific question and design the experiment. With a material science background, I am now fully capable to plan and carry out the biologically oriented research. I am fully independent at setting up and conducting cryo-ET experiments and we were able to test the newest cryo-CLEM setup provided by Leica and Zeiss. I mastered the 3D reconstruction of tomogram datasets with advanced approaches, filtering, etc. I was fully capable of processing the data, model and visualize them. I proved to be able to manage the project and get everything done. The project has achieved most of its objectives and milestones for the period, with relatively minor deviations. We fully succeeded to achieve the deliverables described in WP1 and WP2. The research work packages 3-5 were changed with regard to the fact that the project started almost two years after it was planned. We therefore revised the research objectives and formulated new goals.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Cilia are highly conserved eukaryotic organelles that, with the exception of fungi and higher plants are present in nearly all types of organisms from protists to mammals. Through their motility, sensory, and signaling functions cilia play crucial roles in human physiology and development. Defects in cilia have profound impact on human health. Cilia assembly requires a dedicated protein shuttle, intraflagellar transport (IFT), a bidirectional motility of multi-megadalton protein arrays along ciliary microtubules. Although the overall mechanism of transport is generally described, complete high resolution structural description is necessary for the detailed understanding of this complex system.I propose the in-depth structural analysis of IFT features, train types, cargoes, interactions with the molecular motors in the cilia model organism Chlamydomonas. This approach will bring us both a molecular resolution of incorporated protein complexes and dynamic overview of their relations.The project objectives will be achieved by an elabrated chain of methods based on correlative microscopy, combining the specificity of nanobody labelling with the structure preservation and molecular resolution of cryo-electron tomography. We plan to develop two independent workflows for tracing the IFT features based on the most progressive cryo methods in the cooperation with the project partners.It is anticipated that an detailed ultrastructural 3D analysis of intraflagellar transport will provide new fundamental mechanistic insights into this very important biological process. We also expect an overlap to the whole structural biology field due to the methodical development, which will be universally applicable for another structural studies.

Оригинален текст от CORDIS (на английски).

Участници

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV · MUNCHENКоординаторГермания