H2020Индивидуална стипендия2017–2019

NanoVoltSens · Voltage-sensing nanorods for super-resolution voltage imaging in neurons

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-05-01 → 2019-04-30
Финансиране от ЕС: 185 076 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Полупроводникови нанопръчици се използват като сензори, които се вграждат в мембраната на невроните за визуализиране на електрическото им напрежение. Тази технология помага за по-доброто изучаване на начина, по който мозъкът интегрира сигналите си.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Voltage-sensing nanorods for super-resolution voltage imaging in neurons

In this project we characterized an innovative voltage sensor for neurons that is fundamentally different from the existing voltage sensors. Our sensor is based on targetable voltage-sensing semi-conductor nanorods (vsNRs) that self-insert into the neuronal membrane. The goal of the project was to validate, calibrate and use vsNRs in neurons. We developed vsNPs to be injectable, targetable, and self-insertable into the neuronal membrane such that they could optically and non-invasively report membrane potential at the single particle level at the nanoscale, at multiple sites and across a large field-of-view. We aim to provide a viable and user-friendly voltage-imaging technology that will be widely applicable for the study of signal integration in the brain.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In the last decade, the rapidly developing optical imaging field has significantly improved our understanding of information processing in the brain. Although a number of promising tools have been designed, sensors of membrane potential are lagging behind. In this project we aim to characterize an innovative voltage sensor for neurons that is fundamentally different from the existing ones. Our sensor is based on targetable voltage-sensing semi-conductor nanorods (vsNRs) that self-insert into the neuronal membrane. The rods optically and non-invasively record action potentials at the single particle level, at multiple sites, and across a large field-of-view. Such vsNRs offer unique advantages, including: (1) large voltage sensitivity, (2) ratiometric imaging based on a large spectral shift as function of voltage, (3) very fast response times in the range of ns, (4) very high brightness that affords single-particle detection, and (5) excellent performance in the near-infrared spectral range. The goal of this project is to validate, calibrate, and use vsNRs in neurons, focusing on dendritic spines and dendritic voltage spikes. After initial characterization of vsNRs in neurons, we will take advantage of the exceptional spatial and temporal resolution provided by vsNRs in order to access electrical properties of individual dendritic spines, as well as to make use of the red-shifted emission of vsNRs in order to record simultaneously membrane potential and calcium fluctuations at dendritic branch points. The project is to be accomplished in the laboratory of Dr. Antoine Triller at the IBENS, Paris by Dr. Anastasia Ludwig, who has extensive experience in molecular neurobiology, including analysis of dendritic spine morphology and dynamics.Based on our preliminary data, we are certain to be able to provide within two years a viable and user-friendly voltage-imaging technology that will be widely applicable for the study of signal integration in the brain.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE · ParisКоординаторФранция