MIDAS · Predictive modelling of GPCR druggable allosteric sites
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2017-11-01 → 2019-10-31
- Финансиране от ЕС
- 195 455 €
- Участници
- 1
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
GPCR рецепторите в човешкия геном се анализират чрез компютърни симулации, за да се открият аллостерични сайтове – специални „джобове“ за свързване на лекарства. Това помага за създаването на по-селективни медикаменти с по-малко странични ефекти.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Predictive modelling of GPCR druggable allosteric sites
G protein-coupled receptors (GPCRs) are the most successful druggable targets in the human genome. Up to 60 % of prescription drugs in the European Union act by modulating biological pathways involving GPCRs. GPCRs can be targeted by small molecules acting on allosteric binding sites (ABSs), i.e. pockets spatially distinct from highly conserved orthosteric sites that host endogenous modulators. The design of allosteric modulators (AMs), therefore, represents a viable strategy to achieve more selective drugs with fewer side effects. The discovery of ABSs targeted by AMs in GPCRs is a challenging task that is largely achieved through cost-intensive and time-consuming high-throughput screening campaigns. The recent release of experimental three-dimensional structures of GPCRs in complex with AMs, mainly solved by X-ray crystallography, has opened new opportunities to develop structure-based computer-aided strategies to identify ABSs and screen for novel AMs. MIDAS aimed at developing the first cost-effective and rapid computational methodology based on probe mapping MD simulations for the identification of ABSs in GPCRs by pursuing four objectives: 1) Identification of suitable probes for MD-based co-solvent mapping 2) Development of a computational procedure to identify ABSs within the helical bundle 3) Development of a computational procedure to identify ABSs between helices and lipids 4) Prospective validation of the computational procedures via site-directed mutagenesis experiments
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
G protein coupled-receptors (GPCRs) are the most successful class of druggable targets in the human genome with an estimated 30 to 60 % of prescription drugs in European Union. GPCRs biological state can be modulated by small molecule ligands acting on spatially distinct allosteric binding sites (ABSs) that result more selective and less toxic than drugs binding to orthosteric sites. The discovery of lead compounds targeting ABSs is challenging and to date has largely been achieved through cost-intensive and time-consuming high-throughput screening. The recent release of X-ray structures of GPCRs in the complex with allosteric modulators opens new opportunities to develop structure-based computer aided methodologies to identify ABSs.MIDAS will aim to develop a computational methodology for the search of GPCR ABSs that will include cutting-edge enhanced sampling molecular dynamics simulation methods in cosolvent mapping. The action will solve several current limitations in cosolvent mapping: probe non-specific binding, protein denaturation, low probe sampling, and membrane distortion. MIDAS firstly develop the methodology in retrospective studies, aiming to identify ABSs in the M2, CRF1, P2Y and GCGR receptors for whom crystal structures complexed with allosteric modulators are available. The computational procedures will be developed for identification of extracellular and intracellular ABSs and ABSs at the interface between GPCR helices and lipids. Next, MIDAS will explore the developed protocols in prospective studies using the M3, µ-opipid and CCR5 receptors with available X-ray structures and known ligands. The action envisions the collaboration with experts from both academia and industry and is set to expand scientific, transferable and collaborative skills of the Experience Researcher. The outcome of the action will foster the development of novel health technologies for the discovery of safer drugs targeting membrane proteins.
Оригинален текст от CORDIS (на английски).
Участници
- THE QUEEN'S UNIVERSITY OF BELFAST · BELFASTКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз