H2020Индивидуална стипендия2017–2019

MUSICAL · Chip-based MUSICAL nanoscopy for imaging endocytosis pathways of phage viruses in liver sinusoidal endothelial cells

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-06-09 → 2019-06-08
Финансиране от ЕС: 208 400 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Наноскопията с чипове изследва пътищата, по които фагови вируси навлизат в ендотелните клетки на черния дроб. Високата разделителна способност при живи клетки помага за по-лесна диагностика и разработване на лекарства.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Chip-based MUSICAL nanoscopy for imaging endocytosis pathways of phage viruses in liver sinusoidal endothelial cells

The present state-of-the-art of optical nanoscopy lacks to provide high optical resolution (~50 nm) at high temporal resolution (~1 Hz) over large field of view (> 500 X 500 μm2) in live-cell friendly imaging conditions, such as without special buffer/fluorophores and with minimum photo-toxicity. The development of high throughput nanoscopy using integrated optics and nanoscopy algorithms will allow for high throughput screening of biological samples and pathological samples at significantly lower costs, smaller time scales, and simpler infrastructure needs than electron microscopy. This will enable easier diagnostics and drug development. Ability to image living cells and cell colonies will help in studying disease onset and progression and effect of drugs at the functional level (i.e. cell organelles) inside living cells. In this project, we have delivered the overall objective of achieving ~50 nm optical resolution at small temporal scales (~ seconds) in photochemical environment which is physiologically conducive for in-vivo bio-imaging applications. A secondary objective that was delivered was the exploitation of the illumination patterns supported by photonic chip by computational nanoscopy algorithm MUSICAL to achieve large field-of-view super-resolution live cell imaging. A tertiary objective of training the experienced researcher (ER) for transitioning into faculty position was achieved successfully after the ERC starting grant application 3D-nanoMorph was granted to the ER in 2018. The ERC starting grant started after the completion of this project.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Research in biology is both empowered and limited by the fluorescence imaging technology, which has witnessed a huge leap in resolution limits through super-resolved optical microscopy or also referred as optical nanoscopy. Over the last few decades, numerous optical nanoscopy techniques have been reported for either spatial or temporal resolution enhancement. Nevertheless, the present state-of-the-art of optical nanoscopy lacks to provide high optical resolution (50 nm or better) at high temporal resolution (~1 Hz) over large field of view (> 500 X 500 μm2) in live-cell friendly imaging conditions, such as without special buffer/fluorophores and with minimum photo-toxicity. In this proposal, the aim is to provide ~50 nm optical resolution at significantly small temporal scales (~ seconds) in photochemical environment which is physiologically conducive for in-vivo bio-imaging applications. This is achieved by incorporating complementary knowledge and intra-disciplinary skills on the computational nanoscopy (of the experienced researcher, ER) and chip-based optical nanoscopy technique (developed by the hosting PI). The aim is to apply Multiple Signal Classification algorithm (MUSICAL), a computational nanoscopy algorithm developed by the experienced researcher with the fluctuating illumination provided by waveguide chip-based optical nanoscopy developed at the host university, namely Universite it Tromsø, under the ERC funded project (Nanoscopy, PI: Ahuliwalia). The technique shall be used to image and understand endocytosis transport-highway of phages virus in liver endothelial scavenger cells.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET · TromsoКоординаторНорвегия