H2020Индивидуална стипендия2017–2019

MixAmox · Understanding nitrogen metabolic interactions in mixed anammox-based microbial communities

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-09-01 → 2019-08-31
Финансиране от ЕС: 165 599 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Микробните общности в отпадните води се анализират, за да се разбере как органичният въглерод влияе върху бактериите, превръщащи амонието в азотен газ. Това помага за създаването на по-ефективни пречистни станции, които пестят енергия и намаляват вредните емисии.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Understanding nitrogen metabolic interactions in mixed anammox-based microbial communities

Biological treatment of municipal wastewaters plays a fundamental role in our society by reducing the associated public-health risks and avoiding the pollution of natural resources and ecosystems. Improving current treatment solutions towards more reliable and resource-efficient processes at increasingly stringent discharge limits is a priority of the EU. Successful biological treatments rely on the concerted activity of microorganisms belonging to different functional guilds. As a result, optimized microbial community engineering strategies are required to achieve this goal. Recent years witnessed tremendous advances in our knowledge of the natural nitrogen cycle, challenging our traditional approach to wastewater treatment plants (WWTP) design and operation. In particular, the discovery of the anammox metabolism, catalysing the autotrophic oxidation of ammonium to N2 via nitrite, holds promise to turn the energy balance of WWTPs to neutral or even positive. The overarching goal of the MixAmox project was to advance our fundamental understanding of the principles of microbial selection in anammox-based communities. Specifically, the action focused on the impact of easily degradable organic substrates, a key challenge towards the implementation of anammox processes. Three main objectives were pursued, namely i) characterize the eco-physiological response of different anammox species to easily degradable organic carbon, ii) identify the conditions selecting for high-affinity accompanying N2O reducing guilds as a mean to counteract N2O emissions, and iii) elucidate the role of dissimilatory nitrite reduction to ammonium (DNRA) in biofilm-based anammox processes. To this end, continuous enrichments were combined with meta-proteomics and mathematical modelling, leading to the following conclusions: i) shot-gun meta-proteomics is an appropriate tool for the simultaneous taxonomic and functional characterization of complex microbial communities; ii) among the two tested anammox genera, Candidatus Kuenenia and Ca. Brocadia, the latter has a clear competitive advantage in the presence of organics; iii) long sludge retention times allow for the selective enrichment of clade II N2O reducers; iv) numerical simulations suggest that DNRA defines the nice for anammox in biofilms at high influent organics concentrations.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Improving current biological wastewater treatment plants (WWTPs) towards more reliable and resource-efficient solutions is a priority of the EU (Water Framework Directive). Successful biological treatments rely on the concerted activity of different microorganisms and thus optimized microbial community engineering strategies are required to achieve this goal. Processes based on anammox bacteria hold promise to reduce the energy and resource expenditures of WWTPs. However, our current knowledge of the different microorganisms involved and the environmental factors regulating their interactions in anammox-based microbial communities remains limited. The MixAmox project aims at advancing our fundamental understanding of the metabolic interactions and their implications for process performance in these communities through a multidisciplinary approach based on theoretical and experimental methods combined with cutting-edge molecular techniques and stable isotopes. The obtained results are expected to foster the implementation of anammox systems. Moreover, the developed methodological framework will be directly applicable for the study of any natural or engineered complex microbial community. Hosted at TU Delft, the researched (ML) will develop a strong theoretical background in thermodynamic analysis and mathematical modelling of microbial communities. He will also learn how to design and conduct short and long-term incubations, combined with stable isotopes, targeting the study of metabolic interactions and microbial competition dynamics. ML will spend the secondments at Aalborg University where he will be trained in high-throughput sequencing technologies for the phylogenetic and functional characterization of complex communities. This unique combination of expertise will significantly improve ML’s career perspectives towards his ambition to establish his own research group at the edge of environmental biotechnology and community systems microbiology.

Оригинален текст от CORDIS (на английски).

Участници

TECHNISCHE UNIVERSITEIT DELFT · DelftКоординаторНидерландия