H2020Индивидуална стипендия2017–2019

PFIMDULA · Post-translational modifications during floral initiation: molecular dissection of the UFO-dependent LFY activation

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2017-06-08 → 2019-06-07
Финансиране от ЕС: 185 076 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Молекулярните процеси при образуването на цветове се изследват чрез взаимодействието между протеините LFY и UFO. Разбирането на този механизъм помага за промяна на структурата на растенията, което може да подобри земеделието и продоволствената сигурност.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Post-translational modifications during floral initiation: molecular dissection of the UFO-dependent LFY activation

Issue: Why LEAFY (LFY), a transcriptional factor, activity require interaction with unusual floral organ (UFO), a Skp1-Cullin-F-box E3 ubiquitin ligase complex, to build flowers? Importance for society: Provide insights on the mechanism of LFY activation by UFO, a key step in the making of flowers and hence the continuation of life in flowering plants. Project sheds light on the molecular mechanism of flower formation setting the means to modify at will the architecture of plant inflorescences. The outputs have application in agriculture and potentially impacting social needs on food security. Overall Objectives: WP1. To identify LFY post-translational modifications (PTMs) WP2. To validate ubiquitinated LFY lysine residues and characterize their functional importance WP3. To map LFY-UFO interacting domains and the role of PTMs WP4. To determine LFY interactome in planta

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Flowering plants are the most successful plant group on Earth and provide the basis for most human and animal food. The development of flowers is dependent on a gene regulatory network orchestrated by LEAFY (LFY), the master transcription factor. In Arabidopsis as in other flowering plants, LFY activity is dependent upon its interaction with UFO, a member of the Skp1–Cullin–F-box E3 ubiquitin ligase complex. The goal of this project is to understand why LFY requires interacting with UFO to build flowers. For this, we will apply state-of-the-art technologies including mass-spectrometry, crystallography, biochemistry and plant developmental genetics to identify and characterize the function of Post-Translational Modifications (PTM) on LFY or LFY interacting proteins, with special emphasis on ubiquitination. After identifying key residues involved in the LFY-UFO interaction or targeted by PTM, their functional importance will be assayed in planta. This project should facilitate better understanding of the mechanism of LFY activation. LEAFY and the development of flower will be used here as a model to understand the “activation by degradation” mechanism, still elusive in most organisms and poorly studied in plants. Moreover, understanding the principles governing LFY activation should provide means to modify at will the architecture of plant inflorescences. This project builds on the current background of the applicant in the field of Mass-Spectrometry combined with the outstanding expertise at Grenoble and high-throughput facilities in Structural biology and plant developmental genetics. Notably, the applicant will work within one of the top French Research Centers and in a multidisciplinary environment that will allow the fellow to acquire complementary ‘new knowledge and skills’ in structural biology and developmental genetics. This will re-enforce the fellow’s background and professional maturity for employment opportunities in both academia and industry sectors.

Оригинален текст от CORDIS (на английски).

Участници

COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES · ParisКоординаторФранция