H2020Индивидуална стипендия2018–2020

ARCATOM · Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-10-01 → 2020-12-15
Финансиране от ЕС: 170 122 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Митохондриалното дишане в плодовете на домата се изследва, за да се разбере как се осигуряват енергията и въглеродът за производството на оцветители като ликопена. Тези знания помагат за разработването на стратегии за подобряване на качеството и продуктивността на зеленчуковите култури.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening

Fruit ripening is a highly coordinated developmental process that facilitates animal-driven dispersal of mature seed. Tomato (Solanum lycopersicum) is the main model system for fruit ripening studies at biochemical, genetic and molecular levels. In this economically relevant climacteric fruit, a sudden increase in respiration takes place at the onset of ripening, usually in concert with increased production of ethylene that eventually impacts fruit color, firmness, taste, and flavor. Tomato ripening also involves the differentiation of chloroplasts into chromoplasts that accumulate high levels of β-carotene (pro-vitamin A) and lycopene, carotenoid pigments that provide the characteristic orange and red colors to the ripe fruit. Carotenoids also act as precursors for the synthesis of volatiles that contribute to the characteristic aroma of tomato fruit. Furthermore, dietary carotenoids are powerful health-promoting antioxidants. While a respiratory process supplying ATP has been proposed to function in tomato fruit chromoplasts, it is likely that most ATP and metabolic precursors for plastidial metabolism are imported into chromoplasts. The scientific aim of ARCATOM was to address the specific contribution of mitochondrial respiration to the supply of energy and carbon for the production of carotenoids in tomato fruit during ripening. The project provides novel information into the interactions between primary and secondary metabolism which are still poorly understood. Specifically, the role and regulation of the alternative respiration during fruit ripening, which is still a matter of debate, has been unravelled. The outcomes of this research can be used to design new biotechnological strategies in order to improve vegetable crops productivity and fruit quality.Therefore, this research is aligned with the strategy of Horizon 2020 of the European Commission, in the “Food security, sustainable agriculture and forestry, marine and maritime and inland water research and the bioeconomy”.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Tomato is one of the most economically relevant crops in the world and is the main model system for fruit ripening studies at biochemical, genetic and molecular levels. In this climacteric fruit, a sudden increase in respiration takes place at the onset of ripening, usually in concert with increased production of ethylene that eventually impacts fruit color, firmness, taste, and flavor. During ripening, tomato accumulates high contents of health-promoting carotenoids (pro-vitamin A, antioxidants), which require a high production of carbon precursors and ATP. In this respect, both mitochondrial and chromoplast respiration have been proposed to play important roles in fruit ripening and carotenoid metabolism. The general aim of this project is to determine the contribution of mitochondrial respiration to the supply of energy and carbon for the production of carotenoids in tomato fruit during ripening. In particular, we will perform metabolomics of genetically modified tomatoes with altered mitochondrial and chromoplast respiration at different ripening stages. Furthermore, 13C- and 14C-labelling experiments will be performed to trace the fate of carbon from primary to secondary metabolites. In parallel, we will measure the relative glycolytic and TCA cycle fluxes and, for the first time in tomato, the in vivo activities of mitochondrial electron transport chain pathways by using the 18O fractionation technique. Combining these data with the analysis of transcript and protein levels of the main components of respiratory and carotenoid pathways and mathematical modelling will unveil novel metabolic checkpoints and connections between primary and secondary metabolism during fruit ripening. The generated new insights should contribute to the implementation of new biotechnological approaches to produce fruits with enhanced levels of carotenoids and other health-promoting secondary metabolites.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания