H2020Индивидуална стипендия2018–2020

NAIL · Adsorption and Dynamics of Nucleic Acids on Surfaces in Ionic Liquid Environment

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-03-01 → 2020-02-29
Финансиране от ЕС: 172 800 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Йонните течности и тяхното влияние върху начина, по който ДНК се прикрепя към повърхности, се анализират чрез специални микроскопични техники. Това помага за създаването на по-стабилни биосензори, биоелектроника и микрофлуидни чипове.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Adsorption and Dynamics of Nucleic Acids on Surfaces in Ionic Liquid Environment

The current pool of pharmaceutical excipients is limited to a small number of sugars, amino acids, polymers, salts and detergents. This pool of stabilizing agents can receive a tremendous boost with the inclusion of ionic liquids (ILs). ILs have shown tremendous scope in increasing the stability of nucleic acids, even beyond conventional buffers. In addition ILs have unique properties like low volatility, large electrochemical window, biocompatibility which renders them desirable for applications in bioelectronics, biosensors, and microfluidic chips. To realize this tremendous potential of ILs, NAIL employs this unique combination of IL-DNA systems to understand the effect of IL on DNA adsorption on 2D surfaces. Scanning probe techniques have been used to understand the effect of various ions of the IL on the adsorption behavior of the DNA. The project also explored the role of specific interactions between the IL cation and the DNA bases on the overall persistence length and rigidity of the DNA. In addition to this, the project successfully demonstrates that by tuning the structure and concentration of the IL electrolyte the flexibility of the DNA chain can be controlled

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Nucleic acid storage and stability is of primary importance as it finds uses in material science as nanomachines, biosensors and bioelectronics. The long-term stability and storage of DNA is a major challenge as hydrolytic reactions cause its denaturation when stored in an aqueous environment with conventional buffer solutions. Most of the devices employing nucleic acids require the nucleic acid molecule (generally DNA) to be adsorbed on the surface with a solvent in its immediate vicinity. The search for suitable solvents and electrolytes received a boost with the advent of ionic liquids (ILs). Nucleic acids have showcased enhanced chemical and structural stability in ILs compared to conventional buffer solutions. However, to employ ILs as electrolytes or solvents in biosensors and bioelectronics it is paramount to understand the effect of IL ions on the adsorption and dynamics of DNA molecules on surfaces. The objective can be achieved by employing a synergistic approach of microscopy, spectroscopy and theory. Time lapse scanning force microscopy (SFM) and Tip Enhanced Raman Spectroscopy (TERS) supported by computational studies will give a comprehensive idea in this regard. In essence, the central research objective of NAIL is to understand, the role of the various stabilizing interactions on Nucleic Acids imposed by the ions of Ionic Liquids on the adsorption and dynamics of the former on surfaces. Along with the research objectives, the ultimate goal of the project is to propel me, Sugosh Prabhu to a position of professional independence at the forefront of academic/industrial research by enhancing my innovative potential, diversify my individual competences; and also to open up new avenues in terms of collaboration for me and the host organization.

Оригинален текст от CORDIS (на английски).

Участници

KATHOLIEKE UNIVERSITEIT LEUVEN · LeuvenКоординаторБелгия