H2020Индивидуална стипендия2018–2021

MeloCRISP · Implementation of CRISPR/Cas9 technology in melon to edit fruit ripening and CMV resistant genes

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-05-15 → 2021-03-28
Финансиране от ЕС: 170 122 €
Участници: 1
Схема: MSCA-IF-EF-CAR

Линиите свързват координатора с партньорите.

Накратко на български

Генното редактиране с CRISPR/Cas9 се използва при пъпешите, за да се промени работата на гени, отговорни за узряването на плодовете. Това помага за намаляване на загубите след реколтата и подобряване на качеството на продукцията.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Implementation of CRISPR/Cas9 technology in melon to edit fruit ripening and CMV resistant genes

Worldwide, up to 40% of vegetables and fruits are lost after harvest, which is translated into an economic cost estimated in 4 billion EUR for the EU. The main causes of these loses are mechanical, physiological, pathological, and environmental factors. Among them is fruit ripening, which is a biological process that affects post-harvest durability of fleshy fruits. During ripening the fruit suffers several metabolic changes which affect its softness, colour, flavour and sweetness turning into an attractive fruit for the consumers during a narrow time window. Melon is a crop of high economic importance in Spain, since it is the main melon producer in the EU and the first world exporter. In this scenario, the MeloCRISP project proposed to progress our current understanding on the genomic control underlying fruit ripening using melon as a model species. We studied the role of previously identified candidate genes involved in the regulation of fruit ripening by using the latest technologies in the field of gene editing: ¨CRISPR/Cas9¨. Target genes were edited and the role of the genes was studied by comparison of the fruit ripening behavior of the edited and non-edited plants. Overall, MeloCRISP is a multidisciplinary project in which we studied the genetic control of a trait that affects fruit quality and production. To our knowledge, this is the first time that the CRISPR technology has been implemented on genes involved in agronomically important traits in melon. We have obtained edited plants that validate the role of the target genes in fruit ripening. These results will become a valuable tool for melon breeding programs, which will be able to implement CRISPR/Cas9 technology, and will end up having an impact on the producers and the consumers.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Meeting with global food demand is one of the main priorities in the 2016-17 work programme of H2020. Virus diseases areamong the main causes of crop production losses, being Cucumber mosaic virus (CMV) the virus with broadest host range.On the other hand, post-harvest losses are estimated to account for 15-40% of fruit and vegetable production worldwide. Inthis scenario, several efforts are ongoing to identify genes underlying fruit quality and production such us those related withclimacteric ripening and CMV resistanceMelon (Cucumis melo L.) is one oft he most economically important group of vegetable crops after Solanaceae, and hasemerged as a crop model for important biological traits due to its phenotypic variability and the the availability of genetic andgenomic resources. Previous studies at the host group have identified and mapped genes involved in the regulation ofclimacteric fruit ripening and in a novel mechanism for CMV resistance in this species. MeloCRISP proposes to progress ourcurrent understanding on the genomic control of these important agronomic traits by functionally validating the identifiedcandidate genes. The CRISPR/Cas9 system will be implemented for the first time in this species and used for precise geneediting. In order to delay or impair fruit ripening in climacteric varieties, a gene knockout strategy will be approached. On theother hand, a precise nucleotide substitution in the resistance to CMV region (cmv1) will be performed to generate CMVresistant plantsThe expected outcome is the development of non-transgenic melon CMV resistant plants and varieties with improved fruitquality. This inter-sectorial and multidisciplinary project involves the engagement of a seed industry which will guarantee thetransfer of the results to the seed market, ultimately leading to a high impact in the agriculture and food industry sector.Moreover, the implementation of CRISPR/Cas9 will become a valuable tool for melon breeding programs.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания