H2020Индивидуална стипендия2018–2020

BioIsoK · Mechanisms of K stable isotope fractionation in vertebrates and significance to their energy metabolism

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-10-01 → 2020-09-30
Финансиране от ЕС: 183 455 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Стабилните изотопи на калия се анализират в органи като мускули и черен дроб, за да се проследи транспортът им през клетъчните мембрани. Това помага за по-доброто разбиране на енергийния метаболизъм и физиологията при здрави и болни гръбначни животни.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Mechanisms of K stable isotope fractionation in vertebrates and significance to their energy metabolism

The recent advent of the use of natural stable isotopes of bio-essential metals (e.g., Mg, Ca, Cu…) opened up unprecedented avenues for the study of their cycling in vertebrates and reveal aspects of physiology that remain otherwise unseen. Potassium (K) is the only multi-isotope bio-essential and ubiquitous metal that is so far virtually unexplored in the field of vertebrate physiology, because of specific analytical hindrances with the currently widely available techniques (e.g., multi-collector inductively coupled plasma mass-spectrometry or MC-ICP-MS). Nevertheless, K isotopes show a high potential for the study of physiopathology and metabolism of extant and extinct vertebrates. Potassium represents the major inorganic ion (K+) of the intracellular fluids, contrasting with the actively maintained low concentrations in extracellular fluids. This heterogeneous distribution is maintained by its active transport across cell membranes and is at the basis of a series of vital functions. In the hypothesis that transmembrane transport of K+ is associated with isotope fractionation, the key organs of the K cycle (e.g., muscle, liver or kidney) should significantly contribute to the heterogeneous distribution of K isotopes within the organisms. Furthermore, the current understanding of the K whole-body and cellular cycling in vertebrates suggests that K isotope distribution could notably depend on the metabolic rates of the organism, and therefore inform about allometry or thermo-physiology traits. Such an approach is also of interest for investigating the K cycle in health and disease of extant vertebrates such as human. In order to realize the currently untapped potential of K isotopes for the study of vertebrate biology, the BioIsoK project intended to: a. Develop methods to measure K stable isotope compositions with sufficient accuracy and precision for biological variability to be resolved, using a state-of-the-art prototype of collision/reaction cell double focusing mass spectrometer (CRC-MC-ICP-MS). b. Identify the main mechanisms driving the distribution of K isotopes in modern vertebrates c. Assess the significance of K stable isotopes as indicators of vertebrate energy metabolism

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The reconstruction of physiological and ecological traits of extinct organisms is crucial for apprehending the dynamics of the evolution of species and ecosystems as well as the origins of modern biodiversity. The recent advent of the use of natural stable isotopes of bioessential metals is principally related to the democratization of multi-collector inductively coupled plasma mass spectrometers (MC-ICP-MS). These isotope systems (Mg, Ca, Cu, Fe or Zn) opened up unprecedented perspectives for the study of their cycling in past and present vertebrate organisms and turned out to be precious assets for the unraveling of otherwise inaccessible biological features of fossil organisms, being ecological, behavioral or physiological characteristics.Potassium (K) is a bioessential metal in all vertebrates, where its cycling intensity is notably dependent on their metabolic rates, the later varying itself with thermophysiology from a phylogenetic class to another or with body mass within a given class. Due to its crucial biological functions as well as the observed significant effects of biological processes on its isotope ratios, K isotopes constitute a highly promising novel isotope system for the study of vertebrate metabolism. However, the K stable isotope compositions of vertebrate tissues are currently fairly unexplored, notably due to major technical hindrances, inherent to the existing mass spectrometry technologies. This project aims first to develop a reliable method of K stable isotope analysis using the state-of-the-art “Proteus” prototype MC-ICP-MS implemented with the collision-reaction cell technology and developed by Tim Elliott group and Thermo Fisher®. This method will then be used for analysis of tissues from vertebrates of various classes reared in controlled conditions. This will allow identifying the main mechanisms driving the isotope compositions of vertebrate tissues and assess their potential for the study of vertebrates energy metabolism.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITY OF BRISTOL · BRISTOLКоординаторОбединеното кралство