ELECTRO NEEDLE · In situ stem cell monitoring system based on conductive nanoneedle devices for tracking cell fates in invasive manner
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2018-10-01 → 2020-09-30
- Финансиране от ЕС
- 183 455 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Силициеви наноигли се използват за насочване на стволови клетки, за да се превърнат в нервни клетки. Това помага за разбирането на регенерацията на нервната тъкан при заболявания като Алцхаймер или при увреждания на гръбначния стълб.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
In situ stem cell monitoring system based on conductive nanoneedle devices for tracking cell fates in invasive manner
In recent years there has been an explosion of interest in cell/tissue research, given its promising medical applications in cell-based tissue regeneration, drug testing, and basic research. Nerve tissue repair is an exciting and high impact area of research to pursue as it directly impacts on the quality of human life, because the adult central nerve system cannot be regenerated on its own after trauma or disease, such as Alzheimer's disease, and spinal cord injuries. Guiding nerve tissue regeneration is a timely, exciting, and high impact area of research as it directly impacts on the quality of human life. To handle these concerns, reliable control of stem cell differentiation into nervous tissues is being important. There have been many creative approaches to properly guide the stem cells to differentiate into neural cells, but one of the most promising ones is nanoneedle (nN) arrays. These arrays are known as guiding cell spreading and the dynamic distribution of focal adhesions and cytoskeletal proteins, and further prompt the cells on arrays to cause morphological changes and reduce migration rates. This directly affects stem cells into a neuronal lineage with varying lengths. In this fellowship, we have aimed to present a new microfabrication approach in which a combination of reactive ion etching protocols. With this new fabrication technique, we could produce high-aspect-ratio, nondegradable silicon nanoneedle arrays with tip diameters that can be finely tuned between 20 and 700 nm.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Stem cell-based therapies to cure nerve system disorders using the self-renewal and multilineage differentiation capacities of the transplanted stem cells have been drawing attention during the past decade. Especially, differentiation of mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs) into neural cells are especially investigated since early 2000’s, thanks to their being much less prone to the ethical issues and the risk of developing teratoma. However, the critical challenges are the difficulty in: (i) guiding their proper differentiation to neural cells, and (ii) tracking their fate, distribution, and migration due to the limited tracking methods. In 2015, the Stevens Group at Imperial College London (ICL) developed high-aspect ratio, porous silicon nanoneedles (pSi nNs) for in vitro and in vivo manipulation of cell behaviour. Remarkably, the nNs penetrate the cell membrane but do not damage the nucleus, instead stimulating nuclear condensation (Published in Nat. Mater., ACS Nano, etc.). However, current nNs in the Stevens Group is degradable within 48 hrs which is not ideal for long-term biological studies, especially for detecting/monitoring the cell differentiation during the culture. Recently, the applicant (Dr Hyejeong Seong) newly developed non-porous, solid version of nNs after her joining to the Stevens Group in March 2017. The new nNs exhibited a high stability in cell culture media and buffer solutions, proving their suitability for long-term investigation of cell fate. This provides an ideal framework for manipulating and exploiting cell behaviour for longer periods as a means for understanding differentiation capacity of this promising stem cell source. Furthermore, we’re expecting that the new nNs are modifiable as conductive electronic sensors, byintegrating new nNs with non-cytotoxic electronic devices. Through these devices, cell morphologies and endogenous receptors, will be assayed without invasive immunoassay.
Оригинален текст от CORDIS (на английски).
Участници
- IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE · LondonКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз